English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 55184/89457 (62%)
造訪人次 : 10678311      線上人數 : 21
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61852

    題名: Evidence for mitochondrial localization of betaine aldehyde dehydrogenase in rat liver - purification, characterization and comparison with human cytoplasmic E3 isozyme
    作者: Chern, Ming-kai;Pietruszko, Regina
    貢獻者: 淡江大學生命科學研究所
    關鍵詞: Aldehyde;Betaine;Dehydrogenase;Mitochondria;Rat liver
    日期: 1999-06
    上傳時間: 2013-05-31 11:33:29 (UTC+8)
    出版者: Ottawa: NRC Research Press
    摘要: Betaine aldehyde dehydrogenase has been purified to homogeneity from rat liver mitochondria. The properties of betaine aldehyde dehydrogenase were similar to those of human cytoplasmic E3 isozyme in substrate specificity and kinetic constants for substrates. The primary structure of four tryptic peptides was also similar; only two substitutions, at most, per peptide were observed. Thus, betaine aldehyde dehydrogenase is not a specific enzyme, as formerly believed; activity with betaine aldehyde is a property of aldehyde dehydrogenase (EC, which has broad substrate specificity. Up to the present time the enzyme was thought to be cytoplasmic in mammals. This report establishes, for the first time, mitochondrial subcellular localization for aldehyde dehydrogenase, which dehydrogenates betaine aldehyde, and its colocalization with choline dehydrogenase. Betaine aldehyde dehydrogenation is an important function in the metabolism of choline to betaine, a major osmolyte. Betaine is also important in mammalian organisms as a major methyl group donor and nitrogen source. This is the first purification and characterization of mitochondrial betaine aldehyde dehydrogenase from any mammalian species.
    關聯: Biochemistry and Cell Biology 77, pp.179-187
    顯示於類別:[生命科學研究所] 期刊論文


    檔案 大小格式瀏覽次數



    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋