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    Title: Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials
    Authors: Wang, San-Lang;Chang, Tao-Jen;Liang, Tzu-Wen
    Contributors: 淡江大學生命科學研究所
    Keywords: Antioxidant activity;Chitosanase;Protease;Serratia sp.;Shrimp shell wastes
    Date: 2009-12
    Issue Date: 2013-05-31 11:33:39 (UTC+8)
    Publisher: Dordrecht: Springer Netherlands
    Abstract: A chitosanase and a protease were purified from the culture supernatant of Serratia sp. TKU016 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of the chitosanase and protease determined by SDS–PAGE were approximately 65 and 53 kDa, respectively. The chitosanase was inhibited completely by Mn2+, but the protease was enhanced by all of tested divalent metals. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitosanase and protease were (pH 7, 50°C, pH 6–7, <50°C) and (pH 8–10, 40°C, pH 5–10, <50°C), respectively. SDS (2 mM) had stimulatory effect on TKU016 protease activity. The result demonstrates that TKU016 protease is SDS-resistant protease and probably has a rigid structure. Besides, TKU016 culture supernatant (2% SPP) incubated for 2 days has the highest antioxidant activity, the DPPH scavenging ability was about 76%. With this method, we have shown that shrimp shell wastes can be utilized and it’s effective in the production of enzymes, antioxidants, peptide and reducing sugar, facilitating its potential use in biological applications and functional foods.
    Relation: Biodegradation 21(3), pp.321-333
    DOI: 10.1007/s10532-009-9303-x
    Appears in Collections:[化學學系暨研究所] 期刊論文

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