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    Title: Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008
    Authors: Wang, San-Lang;Lin, Bo-Shyun;Liang, Tzu-Wen;Wang, Chuan-Lu;Wu, Pei-Chen;Liu, Je-Ruei
    Contributors: 淡江大學化學學系
    Keywords: Pseudomonas, chitinase, chitin, shrimp and crab shell wastes
    Date: 2010-06
    Issue Date: 2011-10-15 23:30:40 (UTC+8)
    Publisher: Seoul: The Korea Science and Technology Center
    Abstract: The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaking at 30℃ for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K₂HPO₄, and 0.05% MgSO₄ㆍ7H₂O (pH 7). The TKU008 chitinase was suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation. The molecular mass of the chitinase was estimated to be 40 kDa by SDS-PAGE. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitinase were pH 7, 50℃, pH 6-7, and less than 50℃, respectively. The chitinase was completely inhibited by Mn²+ and Cu²+. The results of peptide mass mapping showed that 11 tryptic peptides of the chitinase were identical to the chitinase CW from Bacillus cereus (GenBank Accession No. gi 45827175) with a 32% sequence coverage.
    Relation: Journal of Microbiology and Biotechnology 20(6), pp.1001-1005
    DOI: 10.4014/jmb.0911.11017
    Appears in Collections:[化學學系暨研究所] 期刊論文

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