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    题名: Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007
    作者: Wang, San-Lang;Wu, Ying-Ying;Liang, Tzu-Wen
    贡献者: 淡江大學化學學系
    日期: 2011-02
    上传时间: 2011-10-15 23:30:13 (UTC+8)
    出版者: Amsterdam: Elsevier BV
    摘要: BSN1, a nattokinase, was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The BSN1 was purified to homogeneity by three-step procedure with a 515-fold increase in specific activity and 12% recovery. The molecular masses of BSN1 determined by SDS-PAGE and gel filtrations were approximately 30 kDa and 28 kDa, respectively. The results of peptide mass mapping showed that four tryptic peptides of BSN1 were identical to the nattokinase from B. subtilis (GenBank accession number gi14422313) with 37% sequence coverage. The N-terminal amino acid sequence of the first 12 amino acids of BSN1 was AQSVPYGISQIK. The optimum pH, optimum temperature, pH stability, and thermal stability of BSN1 were 8, 40°C, pH 4–11, and less than 50°C, respectively. BSN1 was inhibited completely by PMSF, indicating that the BSN1 was a serine protease. Using this method, B. subtilis TKU007 produces a nattokinase/fibrinolytic enzyme and this enzyme may be considered as a new source for thrombolytic agents.
    關聯: New Biotechnology 28(2), pp.196-202
    DOI: 10.1016/j.nbt.2010.09.003
    显示于类别:[化學學系暨研究所] 期刊論文


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