English  |  正體中文  |  简体中文  |  Items with full text/Total items : 56431/90260 (63%)
Visitors : 11697881      Online Users : 53
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61649


    Title: Mechanistic aspect of Co(HAPP)(TFA)2 in DNA bulge-specific recognition
    Authors: 王文竹;Wang, Wen-jwu;Cheng, C. C.;Huang-fu, W. C.;Hung, K. C.;Chen, P. J.;Chen, Y. J.
    Contributors: 淡江大學化學學系
    Date: 2003-06-01
    Issue Date: 2011-10-15 23:25:17 (UTC+8)
    Abstract: A novel octahedral complex CoII(HAPP)(TFA)2 [hexaazaphenantholine‐cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge‐specific probe with single‐strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double‐stranded DNA and low reactivity toward single‐stranded DNA. Metal–HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high‐resolution polyacrylamide gel electrophoresis. In addition, 3′‐phosphoglycolate cleavage products were trapped and analyzed by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4′‐H abstraction of the deoxyribose moiety.
    Relation: Nucleic acid research 31(8), pp.2227-2233
    DOI: 10.1093/nar/gkg323
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

    Files in This Item:

    File SizeFormat
    index.html0KbHTML64View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback