Identification of Paenibacillus sp. 2S-6 and application of its xylanase on biobleaching

doi:10.1016/j.ibiod.2010.12.006

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Title:	Identification of Paenibacillus sp. 2S-6 and application of its xylanase on biobleaching
Authors:	Ko, Chun-han;Tsai, Chung-hung;Tu, Jenn;Yang, Bing-yuan;Hsieh, Dong-lin;Jane, Wann-neng;Shih, Tzenge-lien
Contributors:	淡江大學化學學系
Keywords:	Biobleaching;Bleaching pretreatment;Eucalypt;Kraft pulp;Paenibacillus;Xylanase
Date:	2011-03
Issue Date:	2011-10-15 23:22:33 (UTC+8)
Publisher:	London: Elsevier Ltd
Abstract:	A Paenibacillus sp. strain 2S-6 was isolated from the black liquor of the first brownstock washing stage of kraft pulping process and identified by its 16S rDNA sequence. This bacterial strain utilized a variety of saccharides and polysaccharides as carbon source, but neither lignin nor lipids. Crude xylanase from Paenibacillus sp. 2S-6 was produced in a 5 L laboratory fermenter at 37 °C, pH 7. After 24 h, up to 10.5 IU xylanase per mg of protein in the crude extract of fermentation broth was obtained. After two-stage ultrafiltration, the optimal activity of partially purified xylanase reached 60.51 IU/mg at 50 °C, pH 6. A major band indicating molecular weight of 33 kDa was shown on SDS-PAGE for the partially purified xylanase. After 4 h at 60 °C, 48.99% and 31.25% residual xylanase activities were demonstrated at pH 7 and 9, respectively. Efficacy of its xylanase on the bleaching agent saving was demonstrated by using 5 IU xylanase per gram oven-dried pulp prior to bleaching, referred as biobleaching. Identical levels of brightness and higher levels of viscosity were obtained for the xylanase pretreated eucalypt kraft pulps followed by a 20% reduction of the bleaching agent dosage in the first step of a commercial C70/D30-Eo-D bleaching sequence.
Relation:	International Biodeterioration and Biodegradation 65(2), pp.334-339
DOI:	10.1016/j.ibiod.2010.12.006
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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