Design and characterization of a short HMG-I/DAT1 peptide that binds specifically to the minor groove of DNA

doi:10.1002/jccs.199800093

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/61556

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题名:	Design and characterization of a short HMG-I/DAT1 peptide that binds specifically to the minor groove of DNA
作者:	鄭建中;Cheng, Chien-chung;簡源宏;Jian, Yuan-hung;羅晴容;Lo, Ching-jung;程家維;Cheng, Jya-wei
贡献者:	淡江大學化學學系
关键词:	Minor Groove Binding;Hmg;Footprinting;Ruthenium;Peptide;窄溝結合;停經促性腺激素;腳印分析;釕;胜
日期:	1998-10-01
上传时间:	2013-07-29 13:48:05 (UTC+8)
出版者:	臺北市：中國化學學會
摘要:	High mobility group protein (HMG) is known to be involved in the formation of high order structure of chromatin. HMGs with minor-groove binding ability in the AT-rich DNA region play a vital role in controlling gene transcription activity. In this report, a 18-residue HMG-I/DAT1 chimeric peptide, PRGRPKGKTLREPRGRPY, was designed and synthesized containing two repetitive PRGRP units and a linking peptide, KGKTLRE, as a targeted DNA-binding peptide. The segment PRGRP is derived from HMG-1 while KGKTLRE is from the DAT1 peptide. Using gel-mobility shift assay and 32P-end labeled 27 bp AT-rich DNA, the dissociation constant of this chimeric peptide was found to he 4.7 × 10^(-6) M, that is, l0^4 times stronger than that of the PRGRP segment stand alone (> 10^(-2) M). In addition, the binding constant was found to increase with the length of AT-rich DNA. The possible DNA binding site of the HMG-I/DAT1 chimeric peptide is determined by footprinting experiments using a minor-groove cleaving agent ruthenium(Ⅲ)-Schiff base complex and a 135-bp 32P-5'-end-labeled DNA restriction fragment of Hind Ⅲ/Rsa I from plasmid pBR322 DNA. The major pattern protected by the HMG-I/DAT1 chimeric peptide exhibits a preference for 5'-AAAT-3' of the AT-rich region. Therefore, this novel design HMG-I/DAT1 chimeric peptide possesses not only a high affinity to AT-rich DNA but also the sequence-specific binding in the minor groove of DNA, which may further lead to the design of short synthetic peptides for therapeutic applications
關聯:	Journal of the Chinese Chemical Society=中國化學會會誌 45(5), pp.619-624
DOI:	10.1002/jccs.199800093
显示于类别:	[化學學系暨研究所] 期刊論文

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