Conversion of squid pen by Pseudomonas aeruginosa K187 fermentation for the production of N-acetyl chitooligosaccharides and biofertilizers

doi:10.1016/j.carres.2010.01.025

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/61544

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題名:	Conversion of squid pen by Pseudomonas aeruginosa K187 fermentation for the production of N-acetyl chitooligosaccharides and biofertilizers
作者:	Wang, San-Lang;Hsu, Wan-Han;Liang, Tzu-wen
貢獻者:	淡江大學化學學系
關鍵詞:	Pseudomonas aeruginosa;Squid pen wastes;Deproteinization;N-Acetyl chitooligosaccharides;Biofertilizers
日期:	2010-05
上傳時間:	2011-10-15 23:15:20 (UTC+8)
出版者:	Kidlington: Pergamon
摘要:	Pseudomonas aeruginosa K187, a protease- and chitinase-producing bacterium, exhibited protease and chitinase activity after three and five days of incubation, respectively. The protease and chitinase were both produced by using 1% squid pen powder (SPP) (w/v) as sole carbon and nitrogen source. After fermentation, the deproteinization rate of the recovered squid pen gradually increased up to 68% on the fourth day. After five days of fermentation, the production of GlcNAc, (GlcNAc)2, (GlcNAc)3, (GlcNAc)4 and (GlcNAc)5 were 1.18 mg/mL, 0.76 mg/mL, 1.02 mg/mL, 0.93 mg/mL and 0.90 mg/mL, respectively. The culture supernatant of K187 also exhibited activity of enhancing vegetable growth. For Brassica chinensis Linn treated with the fifth day culture supernatant, the total weight and total length increased up to 529% and 148%, respectively, compared to the control group. With this method, the production of protease, chitinase, N-acetyl chitooligosaccharides and biofertilizers may be useful for biological applications.
關聯:	Carbohydrate Research 345(7), pp.880-885
DOI:	10.1016/j.carres.2010.01.025
顯示於類別:	[化學學系暨研究所] 期刊論文

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