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    题名: Conversion of squid pen by Pseudomonas aeruginosa K187 fermentation for the production of N-acetyl chitooligosaccharides and biofertilizers
    作者: Wang, San-Lang;Hsu, Wan-Han;Liang, Tzu-wen
    贡献者: 淡江大學化學學系
    关键词: Pseudomonas aeruginosa;Squid pen wastes;Deproteinization;N-Acetyl chitooligosaccharides;Biofertilizers
    日期: 2010-05
    上传时间: 2011-10-15 23:15:20 (UTC+8)
    出版者: Kidlington: Pergamon
    摘要: Pseudomonas aeruginosa K187, a protease- and chitinase-producing bacterium, exhibited protease and chitinase activity after three and five days of incubation, respectively. The protease and chitinase were both produced by using 1% squid pen powder (SPP) (w/v) as sole carbon and nitrogen source. After fermentation, the deproteinization rate of the recovered squid pen gradually increased up to 68% on the fourth day. After five days of fermentation, the production of GlcNAc, (GlcNAc)2, (GlcNAc)3, (GlcNAc)4 and (GlcNAc)5 were 1.18 mg/mL, 0.76 mg/mL, 1.02 mg/mL, 0.93 mg/mL and 0.90 mg/mL, respectively. The culture supernatant of K187 also exhibited activity of enhancing vegetable growth. For Brassica chinensis Linn treated with the fifth day culture supernatant, the total weight and total length increased up to 529% and 148%, respectively, compared to the control group. With this method, the production of protease, chitinase, N-acetyl chitooligosaccharides and biofertilizers may be useful for biological applications.
    關聯: Carbohydrate Research 345(7), pp.880-885
    DOI: 10.1016/j.carres.2010.01.025
    显示于类别:[化學學系暨研究所] 期刊論文


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