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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/60549

    Title: Comparison of different metal film thicknesses of COC-substrate polymerase chain reaction chips with single-side and double-side heaters
    Authors: Chung, Yung-chiang;Lai, Li-wei;Yang, Lung-jieh;Liao, Wei-jie
    Contributors: 淡江大學機械與機電工程學系
    Keywords: cyclic olefin copolymers;polymerase chain reaction;double-side heater;thermal deformation temperature;thermal conductivity
    Date: 2010-03
    Issue Date: 2011-10-14 14:15:04 (UTC+8)
    Publisher: Bellingham: S P I E - International Society for Optical Engineering
    Abstract: The polymerase chain reaction PCR chips of different metal film thicknesses were fabricated, and the PCR machine with/without a top heater and the single-side/double-side heater PCR chips were compared. Before fabricating the chips, one simulated the effect of various thin-film thicknesses and the temperature distributions of various powers, and it helped one to find the optimum parameters. The total substrate
    material was cyclic olefin copolymers, and the gold film was fabricated on the substrate by evaporation. The results after PCR for the deoxyri bonucleic acid DNA of an Escherichia coli TG-1 cell were obtained.
    The results of the DNA amplifying 225 bps were 0.84–0.92 ng/l, and these were 78–85% of the result of PCR machine with a top heater. There was no PCR product in the PCR machine without a top heater and single-side PCR chip. The thermal deformation temperature, thermal conductivity, and substrate thickness are the important parameters in a polymer substrate PCR chip, and the thermal deformation temperature is the key parameter.
    Relation: Journal of Micro/Nanolithography, MEMS, and MOEMS, 9(3), 031006(7pages)
    DOI: 10.1117/1.3455371
    Appears in Collections:[Graduate Institute & Department of Mechanical and Electro-Mechanical Engineering] Journal Article

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