In situ real-time investigation of cancer cell photothermolysis mediated by excited gold nanorod surface plasmons

doi:10.1016/j.biomaterials.2010.01.140

機構典藏 > College of Sciences > Graduate Institute & Department of Physics > Journal Article > Item 987654321/58300

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/58300

Title:	In situ real-time investigation of cancer cell photothermolysis mediated by excited gold nanorod surface plasmons
Authors:	Chen, Cheng-Lung;Kuo, Ling-Ru;Chang, Ching-Lin;Hwu, Yeu-Kuang;Huang, Cheng-Kuang;Lee, Shin-Yu;Chen, Kowa;Lin, Su-Jien;Huamg, Jing-Duan;Chen, Yang-Yuan
Contributors:	淡江大學物理學系
Keywords:	Gold;Plasma;Membrane;Laser;Fluorescence
Date:	2010-05
Issue Date:	2011-09-30 22:01:20 (UTC+8)
Publisher:	Amsterdam: Elsevier BV
Abstract:	The photothermolysis of living EMT-6 breast tumor cells triggered by gold nanorods (AuNRs) with two-photon irradiation was conducted in situ and under real-time observation. The morphology and plasma membrane permeability of the cells were key indicators to the phenomena. AuNRs with an aspect ratio of 3.92, and a longitudinal absorption peak at 800 nm were synthesized with a seed-mediated method. The nanorods surfaces were further modified with polystyrenesulfonate (PSS) for biocompatibility. The prepared nanorods displayed excellent two-photon photoluminescence imaging. In situ real-time results revealed cavities internal to the cells were created from thermal explosions triggered by AuNRs localized photothermal effect. The cavitation dynamic is energy dependent and responsible for the perforation or sudden rupture of the plasma membrane. The energy threshold for cell therapy depended significantly on the number of nanorods taken up per cell. For an ingested AuNR cluster quantity N ∼ 10–30 per cell, it is found that energy fluences E larger-than 93 mJ/cm2 led to effective cell destruction in the crumbled form within a very short period. As for a lower energy level E = 18 mJ/cm2 with N ∼ 60–100, a non-instant, but progressive cell deterioration, is observed.
Relation:	Biomaterials 31(14), pp.4104–4112
DOI:	10.1016/j.biomaterials.2010.01.140
Appears in Collections:	[Graduate Institute & Department of Physics] Journal Article

Files in This Item:

File	Description	Size	Format
0142-9612_31(14)p4104-4112.pdf		2716Kb	Adobe PDF	329	View/Open
0142-9612_31(14)p4104-4112.pdf		2716Kb	Adobe PDF	1	View/Open

Loading...