淡江大學機構典藏:Item 987654321/58250
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    题名: Unraveling the Role of the rssC Gene of Serratia marcescens by Atomic Force Microscopy
    作者: Sheu, Bor-ching;Lin, Chih-chen;Fu, Ying-hsien;Lee, Shih-yuan;Lai, Hsin-chih;Wu, Rung-shin;Liu, Chih-hao;Tsai, Jui-chang;Lin, Shi-ming
    贡献者: 淡江大學化學學系
    关键词: Serratia marcescens;atomic force microscopy (AFM);rssC gene;topography
    日期: 2010-11
    上传时间: 2011-09-30 21:20:19 (UTC+8)
    出版者: Cambridge: Cambridge University Press
    摘要: The product and direct role of the rssC gene of Serratia marcescens is unknown. For unraveling the role of the rssC gene, atomic force microscopy has been used to identify the surfaces of intact S. marcescens wild-type CH-1 cells and rssC mutant CH-1ΔC cells. The detailed surface topographies were directly visualized, and quantitative measurements of the physical properties of the membrane structures were provided. CH-1 and CH-1ΔC cells were observed before and after treatment with lysozyme, and their topography-related parameters, e.g., a valley-to-peak distance, mean height, surface roughness, and surface root-mean-square values, were defined and compared. The data obtained suggest that the cellular surface topography of mutant CH-1ΔC becomes rougher and more precipitous than that of wild-type CH-1 cells. Moreover, it was found that, compared with native wild-type CH-1, the cellular surface topography of lysozyme-treated CH-1 was not changed profoundly. The product of the rssC gene is thus predicted to be mainly responsible for fatty-acid biosynthesis of the S. marcescens outer membrane. This study represents the first direct observation of the structural changes in membranes of bacterial mutant cells and offers a new prospect for predicting gene expression in bacterial cells.
    關聯: Microscopy and Microanalysis 16(6), pp.755-763
    DOI: 10.1017/S1431927610093943
    显示于类别:[化學學系暨研究所] 期刊論文

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