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    題名: Significance of the pH-induced conformational changes in the structure of C-reactive protein measured by dual polarization interferometry
    作者: Sheu, Bor-ching;Lin, Yun-han;Lin, Chih-chen;Lee, Adam Shih-yuan;Chang, Wen-chun;Wu, Jung-hsin;TSai, Jui-chang;Lin, Shi-ming
    貢獻者: 淡江大學化學學系
    關鍵詞: C-reactive protein;Dual polarization interferometry;pH;Cardiovascular disease
    日期: 2010-10-15
    上傳時間: 2011-09-30 21:20:15 (UTC+8)
    出版者: Amsterdam: Elsevier BV
    摘要: Emerging evidence indicates that the conformation of C-reactive protein (CRP) plays important roles in human inflammation and cardiovascular disease (CVD). The different conformations in the structure of CRP under different pH conditions remain an important issue to be investigated for explaining various functions of CRP under certain physiologic and pathologic conditions. We directly measured the pH-induced conformational changes in the structure of CRP by dual polarization interferometry (DPI). The CRP was attached to an aldehyde-functionalized DPI sensor chip at a concentration of 50 μg/ml, and attained 2.019 ng/mm2 to form a surface coverage with a 1.71 × 10−14 mol/mm2 CRP monolayer. A pentagonal structure with an average monolayer thickness value of 5.70 ± 0.12 nm and a layer density of 0.374 ± 0.058 g/cm2 was obtained at pH 7.0. Moreover, the DPI biosensor signals directly reflected the considerable structural parameters and phenomena of conformational changes of CRP in a pH range of 2.0–10.0. The results obtained showed that the pentameric structure of CRP might dissociated into monomers or monomer aggregates as the pH shifts toward both acidic and alkaline conditions, but only partial rearrangements of CRP subunits might occur at extremely acidic physiological conditions. Considering the proinflammatory effect and subclinical chronic inflammation, pH-induced conformational changes in the structure of CRP between monomeric and pentameric formations may strongly relate to vascular atherosclerosis and subsequent CVD.
    關聯: Biosensors and Bioelectronics 26(2), pp.822-827
    DOI: 10.1016/j.bios.2010.06.001
    顯示於類別:[化學學系暨研究所] 期刊論文


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