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    題名: Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker
    作者: Chen, Ching-sung;Chang, Ku-ning;Chen, Ying-hua;Lee, Chih-kung;Lee, Bryan Yong-jay;Lee, Adam Shih-yuan
    貢獻者: 淡江大學化學學系
    關鍵詞: Impedance;Label-free;Conductive linker;S100;Point-of-care
    日期: 2011-02-15
    上傳時間: 2011-09-30 21:19:54 (UTC+8)
    出版者: Amsterdam: Elsevier BV
    摘要: We developed a label-free impedance biosensor based on an innovative conductive linker for detecting antibody–antigen interactions. As the often used conventional long chain thiol is a poor conductor, it is not a suitable material for use in a faradaic biosensor. In this study, we adopted a thiophene-based conductive bio-linker to form a self-assembled monolayer and to immobilize the bio-molecules. We used cyclic voltammetry and impedance spectroscopy to verify the enhanced conductivity properties. Results showed that the electron transfer resistance of this new conductive linker was 3 orders of a magnitude lower than for a case using a conventional long chain thiol linker. With the decreased impedance (i.e. increased faradaic current), we can obtain a higher signal/noise ratio such that the detection limit is improved. Using fluorescence microscopy, we verified that our new conductive linker has a protein immobilization capability similar to a conventional long chain thiol linker. Also, using S100 proteins, we verified the protein interaction detection capability of our system. Our obtained results showed a linear dynamic range from 10 ng/ml to 10 μg/ml and a detection limit of 10 ng/ml. With our new conductive linker, an electrochemical impedance biosensor shows great potential to be used for point-of-care applications.
    關聯: Biosensors and Bioelectronics 26(6), pp.3072-3076
    DOI: 10.1016/j.bios.2010.11.051
    顯示於類別:[化學學系暨研究所] 期刊論文


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