淡江大學機構典藏:Item 987654321/58243
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    Title: Electroporation for three commonly used yeast strains for two-hybrid screening experiments
    Authors: Lin, Hsin-ying;Lin, Sey-en;Chien, Su-fang;Chern, Ming-kai
    Contributors: 淡江大學化學學系
    Date: 2011-09
    Issue Date: 2011-09-30 21:19:41 (UTC+8)
    Publisher: Philadelphia: Elsevier Inc.
    Abstract: The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
    Relation: Analytical Biochemistry 416(1), pp.117-119
    DOI: 10.1016/j.ab.2011.04.038
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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