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    题名: Electroporation for three commonly used yeast strains for two-hybrid screening experiments
    作者: Lin, Hsin-ying;Lin, Sey-en;Chien, Su-fang;Chern, Ming-kai
    贡献者: 淡江大學化學學系
    日期: 2011-09
    上传时间: 2011-09-30 21:19:41 (UTC+8)
    出版者: Philadelphia: Elsevier Inc.
    摘要: The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
    關聯: Analytical Biochemistry 416(1), pp.117-119
    DOI: 10.1016/j.ab.2011.04.038
    显示于类别:[化學學系暨研究所] 期刊論文


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