The aim of this study was to determine the synergistic effect of nisin and green onion extract (GOE) on the inhibition of Listeria monocytogenes cocktail found in full-fat milk (M1), fat-free milk (M2), nano-calcium milk (M3) and nano-iron milk (M4). GOE at concentrations of 2, 5 and 8% revealed significant antilisterial effect against L. monocytogenes cocktail in all retail milk. The addition of nisin at concentrations of 75, 150, 225 and 300 IU/mL also showed a good antilisterial effect compared with the control group. Combination of GOE (2 and 5%) and nisin (75, 150, 225 and 300 IU/mL) resulted in synergistic antilisterial activity in all samples tested (M1∼M4) at 14 days. The synergistic antilisterial ability shows the potential application of combining GOE and nisin as an antilisterial agent in the food industry.
