Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization

doi:10.1163/156856208X396047

淡江大學機構典藏 > 工學院 > 化學工程與材料工程學系暨研究所 > 期刊論文 > Item 987654321/53688

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题名:	Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization
作者:	Lin, Dar-Jong;Lin, Dong-Tsamn;Young, Tai-Horng;Chen, Tsung-Chin;Chang, Hsu-Hsien;Cheng, Liao-Ping
贡献者:	淡江大學化學工程與材料工程學系
关键词:	Poly(vinylidene fluoride) membrane;immobilization;DNA
日期:	2009-12
上传时间:	2011-05-20 09:55:28 (UTC+8)
出版者:	Abingdon: Taylor & Francis Ltd.
摘要:	Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm2, which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 μg/cm2. Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.
關聯:	Journal of Biomaterials Science 20(13), pp.1943-1959
DOI:	10.1163/156856208X396047
显示于类别:	[化學工程與材料工程學系暨研究所] 期刊論文

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