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    Title: Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization
    Authors: Lin, Dar-Jong;Lin, Dong-Tsamn;Young, Tai-Horng;Chen, Tsung-Chin;Chang, Hsu-Hsien;Cheng, Liao-Ping
    Contributors: 淡江大學化學工程與材料工程學系
    Keywords: Poly(vinylidene fluoride) membrane;immobilization;DNA
    Date: 2009-12
    Issue Date: 2011-05-20 09:55:28 (UTC+8)
    Publisher: Abingdon: Taylor & Francis Ltd.
    Abstract: Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm2, which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 μg/cm2. Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.
    Relation: Journal of Biomaterials Science 20(13), pp.1943-1959
    DOI: 10.1163/156856208X396047
    Appears in Collections:[Graduate Institute & Department of Chemical and Materials Engineering] Journal Article

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