本研究以恆壓過濾系統，採透析過濾操作方法移除玻尿酸溶液中之蛋白質溶菌酶，探討在電解質種類及濃度、攪拌速度、進料溶液濃度、透析體積及透析液電解質濃度下，對濾速、過濾阻力、玻尿酸/溶菌酶分離效率之影響。 研究結果顯示，透析過濾玻尿酸與溶菌酶雙成分時，電解質離子強度越大，造成玻尿酸分子脫水效應使HA堆積形態越緊密，濾速相對較低；而電解質造成溶菌酶與薄膜之間的靜電作用力降低，使溶菌酶較不易吸附於膜面上，較易通過薄膜，提高移除效率。加入攪拌可有效降低過濾阻力而提高濾速。隨著透析過濾操作之進行，若透析液中電解質濃度持續增大，對溶菌酶之移除率有顯著改善。 This study investigated the effect of electrolytes on removal of protein(lysozyme,LY) from hyaluronic acid(HA) solution with using diafiltration in a dead-end stirred cell. The flux behavior, filtration resistance and HA/LY separation efficiency were discussed under various operating parameters such as electrolyte types, electrolyte concentrations, stirred rates, feed concentrations and diavolumes. Experimental results showed that the permeate flux decreased with the increase of ion strength of electrolyte which caused the HA molecules to form a more compact deposited layer on membrane surface. Meanwhile, the LY molecules did not aggregate because the surface charge of LY was covered by the electrode ion strength. The isolated LY molecules can pass through the membrane more smoothly. Therefore, the reduction of LY from the mixture solution of HA/LY increased with in creasing the ion strength, the permeate flux can be effectively enhanced by the addition of stir that disturbed the polarization layer of HA and reduced the filtration resistance. In diafiltrating the HA/LY solution, the step increase of electrolyte concentration in dialyate solution has significant.
