啤酒酵母菌JHD1p去甲基酶之選殖及專一性探討

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题名:	啤酒酵母菌JHD1p去甲基酶之選殖及專一性探討
其它题名:	Cloning and specificity of saccharomyces cerevisiae JHD1p demethylase.
作者:	莊登發;Chuang, Teng-fa
贡献者:	淡江大學生命科學研究所碩士班陳銘凱;Chern, Ming-kai
关键词:	啤酒酵母菌;去甲基酶;JHD1p;Saccharomyces cerevisiae;demethylase;JHD1p
日期:	2010
上传时间:	2010-09-23 16:10:25 (UTC+8)
摘要:	染色體的基本組成單元是由H2A、H2B、H3、H4各二分子所組成的核心組蛋白纏繞著146 鹼基對DNA而成。核心組蛋白的共價修飾藉由貢獻額外的遺傳訊息來調節基因組的功能。現階段，組蛋白常見的共價修飾之一是甲基化。近年來，確定甲基化是具有可逆性的，因此發現了另一種機動性組蛋白共價修飾，即去甲基化。所以，組蛋白的共價修飾在基因表現的遺傳控制上扮演一個極關鍵的角色。本研究探討啤酒酵母菌去甲基酶JHD1p的受質專一性研究，基於報導在酵母菌的胞外去甲基酶JHD1p為具活性的酵素，而且大部份研究都以組蛋白上的受質為主要研究目標，所以本實驗想利用啤酒酵母菌去甲基酶JHD1p的胞外活性測試，尋找是否還有非組蛋白的功能性受質存在。 The basic unit of chromatin consists of 146 bps of DNA wrapped around a histone octamer, which is composed of two copies of each of the four core histones: H2A, H2B, H3 and H4. The covalent modification of core histones modulates genome function by contributing additional epigenetic information. At this stage, one of the common covalent histone modification is methylation. In recent years , methylation is determined to be reversible and another dynamic group protein covalent modification, demethylation, is established. Therefore,the covalent modification of histones plays a pivotal role in the epigenetic control of gene expression. In this study, substrate specificity of Saccharomyces cerevisiae JHD1p demethylase is studied. Based on the information known from literature, Saccharomyces cerevisiae JHD1p demethylase is an enzyme with activity in vitro, and most of the studies have focused on the substrate role of histone proteins as the main research goal. Nevertheless, this study would like to use the in vitro activity of enzyme JHD1p to find out whether there is any functional non-histone substrates existing in Saccharomyces cerevisiae.
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