Serratia marcescens TKU011生產靈菌紅素之條件與定性

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题名:	Serratia marcescens TKU011生產靈菌紅素之條件與定性
其它题名:	Production and characterization of prodigiosins from serratia marcescens TKU011
作者:	王振宇;Wang, Chen-yu
贡献者:	淡江大學生命科學研究所碩士班王三郎
关键词:	沙雷氏菌;烏賊軟骨粉末;靈菌紅素;Serratia marcescens;squid pen powder;prodigiosin
日期:	2010
上传时间:	2010-09-23 16:10:07 (UTC+8)
摘要:	Serratia marcescens TKU011 係一株以蝦頭殼粉末作為唯一碳/氮源之蛋白酶及幾丁聚醣酶生產菌。本研究發現以烏賊軟骨粉末（SPP）作為碳/氮源時具有可生產紅色色素-靈菌紅素（prodigiosin）的能力，菌株醱酵產生之紅色色素經分離純化後，藉由1H-NMR 及ESI-MS 確認合成之靈菌紅素屬於prodigiosin。TKU011 生產靈菌紅素之較適培養為含有 1.5%烏賊軟骨粉末、0.1% K2HPO4 及0.05% MgSO4〃7H2O 之50 mL 液態培養基（pH 7~9）於30℃振盪培養一天後接著移到25℃繼續培養兩天，靈菌紅素產量可達0.978 mg/mL。醱酵上清液經試驗推測亦含有脂胜肽類生物界面活性劑沙雷濕潤素（serrawettin），表面張力值隨著靈菌紅素的產量增加而降低。 A protease- and chitosanase-producing strain, Serratia marcescens TKU011, was cultivated by using shrimp-head powder（SHP）as the sole carbon/nitrogen source. In this study, a prodigiosin-like pigment was produced from TKU011 by using squid pen powder（SPP）as the sole carbon/nitrogen source. prodigiosin（PG）is one of prodigiosin-like pigments produced by S.marcescens TKU011 and was purified and characterized by1H-NMR and ESI-MS to determine its chemical structure and molecular weight. The optimized conditions for prodigiosin production were cells used to inoculate in 50mL of medium containing 1.5% SPP, 0.1% K2HPO4, 0.05% MgSO4．7H2O（pH7~9）shaken at 30℃ for one day and then cultivated at 25℃ for two days. The production of PG reached 0.978 mg/mL. Besides, TKU011 culture supernatant also contained the lipopeptide biosurfactant（serrawettin）, and the production of serrawettin increased with PG production.
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