淡江大學機構典藏:Item 987654321/51811
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 64182/96956 (66%)
Visitors : 11219687      Online Users : 22155
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/51811


    Title: Burkholderia cepacia TKU025 生產酪胺酸酶抑制劑之條件及特性分析
    Other Titles: Production and characterization of tyrosinase inhibitors from Burkholderia cepacia TKU025
    Authors: 李昀真;Lee, Yun-chen
    Contributors: 淡江大學生命科學研究所碩士班
    王三郎;Wang, San-lang
    Keywords: Burkholderia cepacia;酪胺酸酶抑制劑;抗菌;Burkholderia cepacia;tyrosinase inhibitor;antimicrobial
    Date: 2010
    Issue Date: 2010-09-23 16:10:03 (UTC+8)
    Abstract: Burkholderia cepacia TKU025係以烏賊軟骨粉為唯一碳/氮源，篩選自台灣北部土壤之一株酪胺酸酶生產菌，經鑑定為Burkholderia cepacia，是一非葡萄糖發酵之革蘭氏陰性菌。B. cepacia TKU025生產酪胺酸酶抑制劑之較適培養條件為含有1.0 % 烏賊軟骨、0.1 % K2HPO4及0.05 % MgSO4．7H2O（pH7）之25 mL液態培養基於30 °C振盪培養4天。
    發酵上清液經乙酸乙酯去除脂溶成分、後續使用水層部分進行Sephacryl S-100以及薄膜層析法(thin layer chromatography)等步驟，進行抑制劑之分離純化。此抑制劑有非常良好之熱安定性及pH安定性，於100 °C及pH 2-12反應下，其活性均不受影響。
    此外，經由Sephacryl S-100層析，所得初步分離之抑制劑對E. coli 及B. cereus TKU006之生長具有顯著抑制活性，兩者抑制效果最高都達到95 %以上。
    The tyrosinase inhibitor producing strain, Burkholderia cepacia TKU025 was isolated from the soil in the northern Taiwan by using squid pen powder (SPP) as the sole carbon/nitrogen source and identified as the Burkholderia cepacia. The strain was a non-lactose-fermenting and Gram-negative bacteria. The optimal conditions for tyrosinase inhibitor production were cells used to inoculate in 25 mL of medium containing 1.0% SPP, 0.1% K2HPO4 and 0.05% MgSO4．7H2O（pH7）and shaken at 30 °C for 4 days.
    The tyrosinase inhibitor was purified by ethyl acetate, chromatography of Sepharose S-100 and thin-layer chromatography (tlc). The tyrosinase inhibitor had broad thermal stability and pH stability. It maintained it’s initial activity at 100 °C and pH 2-12, respectively.
    Besides, the purified inhibitor by Sepharose S-100 chromatography had antimicrobial activity against E. coli and B. cereusTKU006. Both the best inhibition were more than 95 %.
    DOI: 10.6846%2fTKU.2010.00942
    Appears in Collections:[Graduate Institue of Life Sciences] Thesis

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML597View/Open
    index.html0KbHTML274View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback