Burkholderia cepacia TKU025係以烏賊軟骨粉為唯一碳/氮源，篩選自台灣北部土壤之一株酪胺酸酶生產菌，經鑑定為Burkholderia cepacia，是一非葡萄糖發酵之革蘭氏陰性菌。B. cepacia TKU025生產酪胺酸酶抑制劑之較適培養條件為含有1.0 % 烏賊軟骨、0.1 % K2HPO4及0.05 % MgSO4．7H2O（pH7）之25 mL液態培養基於30 °C振盪培養4天。
發酵上清液經乙酸乙酯去除脂溶成分、後續使用水層部分進行Sephacryl S-100以及薄膜層析法(thin layer chromatography)等步驟，進行抑制劑之分離純化。此抑制劑有非常良好之熱安定性及pH安定性，於100 °C及pH 2-12反應下，其活性均不受影響。
此外，經由Sephacryl S-100層析，所得初步分離之抑制劑對E. coli 及B. cereus TKU006之生長具有顯著抑制活性，兩者抑制效果最高都達到95 %以上。 The tyrosinase inhibitor producing strain, Burkholderia cepacia TKU025 was isolated from the soil in the northern Taiwan by using squid pen powder (SPP) as the sole carbon/nitrogen source and identified as the Burkholderia cepacia. The strain was a non-lactose-fermenting and Gram-negative bacteria. The optimal conditions for tyrosinase inhibitor production were cells used to inoculate in 25 mL of medium containing 1.0% SPP, 0.1% K2HPO4 and 0.05% MgSO4．7H2O（pH7）and shaken at 30 °C for 4 days.
The tyrosinase inhibitor was purified by ethyl acetate, chromatography of Sepharose S-100 and thin-layer chromatography (tlc). The tyrosinase inhibitor had broad thermal stability and pH stability. It maintained it’s initial activity at 100 °C and pH 2-12, respectively.
Besides, the purified inhibitor by Sepharose S-100 chromatography had antimicrobial activity against E. coli and B. cereusTKU006. Both the best inhibition were more than 95 %.
