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    Title: Paenibacillus sp. TKU023生產胞外多醣之條件及特性分析
    Other Titles: Production and characterization of exopolysaccharides from paenibacillus sp. TKU023.
    Authors: 黃梓晃;Huang, Tzu-huang
    Contributors: 淡江大學生命科學研究所碩士班
    王三郎
    Keywords: Paenibacillus sp.;胞外多醣;烏賊軟骨粉;Paenibacillus sp.;exopolysaccharide;squid pen powder
    Date: 2010
    Issue Date: 2010-09-23 16:09:52 (UTC+8)
    Abstract: TKU023係以烏賊軟骨粉為唯一碳/氮源,篩選自台灣北部土壤之一株胞外多醣及生物界面活性劑生產菌,經鑑定為Paenibacillus sp.。TKU023生產胞外多醣之較適培養條件為含有1% 烏賊軟骨、0.1% K2HPO4及0.05% MgSO4.7H2O之50 mL液態培養基(pH 7.32)於37℃振盪培養5天。
    TKU023發酵所得上清液經加熱(121℃、20 min)、脫色後可得粗胞外多醣(1.7631 g),再將粗胞外多醣利用Sevag reagent去蛋白、酵素水解、透析後,可將胞外多醣分解成寡醣類物質,利用核磁共振(NMR)光譜分析水解後之寡醣結構,發現其1H、13C譜與麥芽糖之1H、13C譜相似,可推測胞外多醣經酵素水解後可得部分麥芽糖之單體。
    此外以烏賊軟骨粉做為發酵碳/氮源 ,利用TKU023在不同培養體積下培養1~6天,發現培養體積50 mL在培養第5天所得發酵上清液具有較高的DPPH清除能力(77%)及較佳的總酚含量、還原力、螯合亞鐵離子能力。
    In this study, an exopolysaccharide (EPS) and biosurfactant producing strain, was isolated from the soil in the northern Taiwan by using squid pen powder (SPP) as the sole carbon/nitrogen source and identified as Paenibacillus sp.. The optimal conditions for the production of the EPS were cells used to inoculate into 50 mL medium containing 0.5% squid pen powder (SPP), 0.1% K2HPO4 and 0.05% MgSO4.7H2O (pH 7.32)followed by culture at 37℃ for 5 days.
    The crude EPS (1.7631 g) was obtained from the culture supernatant by autoclave (121℃ , 20 min) and bleaching and then the crude EPS followed by deproteinization with Sevag reagent. The deproteinized EPS was hydrolyzed to oligosaccharides by enzyme and dialyzed with water. The oligosaccharides were analyzed by using nuclear magnetic resonance (NMR). The 1H and 13C NMR spectrometric results suggest that EPS hydrolyzates contained maltose as oligomeric building subunit.
    Additionally, Paenibacillus sp. TKU023 was cultivated with various culture volumes for 1~6 days by using squid pen powder as the sole carbon/nitrogen sources. The results indicated that the maximum DPPH free radical scavenging ability and better total phenolic contents, reducing activity, and Fe2+ chelating ability was found at the 5th day by using the medium volume of 50 mL.
    Appears in Collections:[Graduate Institue of Life Sciences] Thesis

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