淡江大學機構典藏:Item 987654321/51806
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 54924/89265 (62%)
造訪人次 : 10601785      線上人數 : 26
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/51806


    題名: Bacillus licheniformis TKU004生產生物界面活性劑之條件與特性分析
    其他題名: Production and characterization of biosurfactants from Bacillus licheniformis TKU004
    作者: 蔣宗儒;Chiang, Tsung-ju
    貢獻者: 淡江大學生命科學研究所碩士班
    王三郎
    關鍵詞: Bacillus licheniformis;Biosurfactant;Surface tension;Bacillus licheniformis;Biosurfactant;Surface tension
    日期: 2010
    上傳時間: 2010-09-23 16:09:49 (UTC+8)
    摘要: 本研究所篩選TKU004這株生物界面活性劑生產菌,經由16SrDNA序列比對及API試驗鑑定為Bacillus licheniformis 。
    B. licheniformis TKU004 生產生物界面活性劑之較適培養條件為含有1%烏賊軟骨粉、0.1% K2HPO4 及0.05% MgSO4.7H2O之50 mL液態培養基 (pH 7)充填於250 mL 通氣錐形瓶並經滅菌20分鐘後,進行接菌並於37℃搖瓶(150 rpm)培養1天。所得醱酵上清液,經酸沈澱、乙酸乙酯萃取、甲醇萃取等步驟,進行生物界面活性劑之純化,其產量為0.551 g/L。TKU004生產之生物界面活性劑能降低水的表面張力至25.42 mN/m,乳化指數(E24)為70%,而且熱穩定性高,不會受高溫影響而失活。此生物界面活性劑之pH 安定性、鹽度安定性分別為pH>7、鹽濃度<2 %。
    此外,2 mg/mL之生物界面活性劑對Fusarium oxysporun生長具有40%抑制效果。
    The biosurfactant producing strain, Bacillus sp. TKU004, was identified further as Bacillus licheniformis by 16S rDNA gene sequencing and API(Analyitcal Profile Index)tests.
    The optimized culture condition for biosurfactant production was composed of 1% squid pen powder (SPP), 0.1% K2HPO4, 0.05% MgSO4.7H2O (pH 7),with autoclave treatment for 20 min, afterward, TKU004 was incubated in 50 mL of above liquid medium in an areation flask (250 mL) and kept shaking at 37℃ for one days.
    The biosurfactant was extracted from the culture supernatant by acid precipitation, extraction of ethyl acetate and methanol .The yields of biosurfactant was 0.551g/L. TKU004 biosurfactant could reduce surface tension of water from 72 to 29 dyne/cm.The emulsification index against machine oil was 80%.The biosurfactant had good thermal stability and was resistant to higher temperatures. The pH stability and salinity stability were pH>7 and salinity <2%, respectively.
    Besides, 2 mg/mL of the biosurfactant had 40% antifungal activity against mycelia growth of Fusarium oxysporun.
    DOI: 10.6846%2fTKU.2010.00644
    顯示於類別:[生命科學研究所] 學位論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML548檢視/開啟
    index.html0KbHTML43檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋