Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture

doi:10.1163/156856209X426574

淡江大學機構典藏 > 工學院 > 化學工程與材料工程學系暨研究所 > 期刊論文 > Item 987654321/50495

請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/50495

題名:	Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture
作者:	Young, Tai-horng;Lin, Ui-hsiang;Lin, Dar-jong;Chang, Hsu-hsien;鄭廖平;Cheng, Liao-ping
貢獻者:	淡江大學化學工程與材料工程學系
關鍵詞:	POLY(VINYLIDENE FLUORIDE);MEMBRANE;IMMOBILIZATION;L-LYSINE;NEURON;CULTURE
日期:	2009-03-01
上傳時間:	2010-08-09 18:20:50 (UTC+8)
出版者:	Brill Academic Publishers
摘要:	Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring-opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm<sup>2</sup>. This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine, the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/cm<sup>2</sup>, a value considerably higher than reported in the literature using other immobilization methods. Furthermore, neurons were cultured on L-lysine-immobilized PVDF membranes. The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish.
關聯:	Journal of Biomaterials Science 20(5-6), pp.703-720
DOI:	10.1163/156856209X426574
顯示於類別:	[化學工程與材料工程學系暨研究所] 期刊論文

文件中的檔案:

檔案	大小	格式	瀏覽次數
0920-5063_20(5-6)p703-720.pdf	1801Kb	Adobe PDF	357	檢視/開啟

在機構典藏中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....