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    题名: Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture
    作者: Young, Tai-horng;Lin, Ui-hsiang;Lin, Dar-jong;Chang, Hsu-hsien;鄭廖平;Cheng, Liao-ping
    贡献者: 淡江大學化學工程與材料工程學系
    关键词: POLY(VINYLIDENE FLUORIDE);MEMBRANE;IMMOBILIZATION;L-LYSINE;NEURON;CULTURE
    日期: 2009-03-01
    上传时间: 2010-08-09 18:20:50 (UTC+8)
    出版者: Brill Academic Publishers
    摘要: Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring-opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm<sup>2</sup>. This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine, the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/cm<sup>2</sup>, a value considerably higher than reported in the literature using other immobilization methods. Furthermore, neurons were cultured on L-lysine-immobilized PVDF membranes. The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish.
    關聯: Journal of Biomaterials Science 20(5-6), pp.703-720
    DOI: 10.1163/156856209X426574
    显示于类别:[化學工程與材料工程學系暨研究所] 期刊論文

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