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    Title: Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture
    Authors: Young, Tai-horng;Lu, Jui-nan;Lin, Dar-jong;Chang, Chi-lin;Chang, Hsu-hsien;Cheng, Liao-ping
    Contributors: 淡江大學化學工程與材料工程學系
    Keywords: Poly(vinylidene fluoride);Porous;Membrane;Immobilization;l-lysine
    Date: 2008-12
    Issue Date: 2010-08-09 18:01:41 (UTC+8)
    Publisher: Amsterdam: Elsevier BV
    Abstract: Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion-precipitation of a casting solution in coagulation baths of different strengths. Onto the membrane surface, an amino acid (l-lysine) was immobilized by a dual-step chemical process. First, the membrane was grafted with poly(acrylic acid) (PAA) by means of plasma-induced free radical polymerization. Then, l-lysine was covalently bonded to the as-grafted PAA chains with the aid of a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC). The highest attainable graft yield of PAA on PVDF membrane reached up to 0.64 mg/cm2. For immobilization of l-lysine on the membrane, the yields were found to depend on factors, such as concentration of EDC, activation time, and pH value. The maximal attainable immobilization yield was 0.65 μg/cm2. Furthermore, pheochromocytoma (PC12) cells were cultured on l-lysine/PAA/PVDF membranes. It was found that both the amount of l-lysine on the membrane and the surface structure had a marked influence on the cell activity. Thus, the present results could be useful for the development of strategies to promote the re-growth and regeneration of tissue in the nervous system.
    Relation: Desalination 234(1-3), pp. 134-143
    DOI: 10.1016/j.desal.2007.09.079
    Appears in Collections:[化學工程與材料工程學系暨研究所] 期刊論文

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