Propolin H from Taiwanese propolis induces G1 arrest in human lung carcinoma cells

doi:10.1021/jf070201n

機構典藏 > College of Sciences > Graduate Institute & Department of Chemistry > Journal Article > Item 987654321/50379

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Title:	Propolin H from Taiwanese propolis induces G1 arrest in human lung carcinoma cells
Authors:	Weng, Meng-shih;Liao, Chiung-ho;Chen, Chia-nan;吳嘉麗;Wu, Chia-li;Lin, Jen-kun
Contributors:	淡江大學化學學系
Keywords:	Propolis;propolin H;lung carcinoma cell;cell cycle;p21Waf1/Cip1
Date:	2007-06-01
Issue Date:	2010-08-09 16:26:40 (UTC+8)
Publisher:	American Chemical Society (ACS)
Abstract:	Propolis, a natural product collected by honeybee, has been reported to exert a wide spectrum of biological functions. In this study, we have isolated a novel component, namely, propolin H, and investigated its effects in human carcinoma cells. Propolin H inhibited the proliferation of human lung carcinoma cell lines in MTT assay, and a significant G1 arrest was observed to occur in a dose-dependent manner at 24 h of exposure in H460 cells. After treatment with propolin H in H460 cells, the content of the CDK inhibitor p21Waf1/Cip1 protein increased in correlation with the elevation in p53 levels. Western blot analysis of G1 regulatory proteins further revealed a decrease in cyclin-dependent kinase 2 (CDK2) and CDK4 and an increase in cyclin E. The CDKs kinase activities assay showed that propolin H has inhibited CDK2 and CDK4 kinase activities. Accordingly, coimmunoprecipitations revealed an increased association of both CDK2 and CDK4 immunoreactive protein with the p21Waf1/Cip1 protein complex under propolin H-treated conditions. Additionally, we found that propolin H enhanced the expression of p21Waf1/Cip1 in p53-mutant and p53-null lung carcinoma cell lines, following the induction of G1 arrest. Together, these findings suggest that the induction of p21Waf1/Cip1 expression occurred through p53-dependent and -independent pathways in propolin H-treated cells. Propolin H exerts its significantly growth inhibitory effects and may have therapeutic applications.
Relation:	Journal of Agricultural and Food Chemistry 55(13), pp.5289-5298
DOI:	10.1021/jf070201n
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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