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    Title: Single-step protein purification by back flush in ion exchange chromatography
    Authors: Chern, Ming-kai;Shiah, Wei-jyh;Chen, Jyun-jie;Tsai, Tzung-you;Lin, Hsin-yin;Liu, Chien-wei
    Contributors: 淡江大學生命科學研究所
    Date: 2009-09
    Issue Date: 2010-08-09 15:49:50 (UTC+8)
    Publisher: Philadelphia: Elsevier Inc.
    Abstract: Ion exchange chromatography, one of the major procedures for protein purification, seldom provides single-step purification due to a lack of specific affinity. In this work, a novel and simple method called “back flush” (i.e., reversing the flow direction of elution relative to that of sample loading) was developed to achieve single-step purification on an ion exchanger. Tips for the conditions and operation by back flush are presented. Our study demonstrates, for the first time, the feasibility and dramatic improvement for protein purification by the back-flush method.
    Relation: Analytical Biochemistry 392(2), pp.174-176
    DOI: 10.1016/j.ab.2009.05.045
    Appears in Collections:[化學學系暨研究所] 期刊論文

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