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    题名: The Micro Patterning of Glutaraldehyde (GA)-Crosslinked Gelatin And Its Application To Cell-Culture
    作者: Yang, Lung-jieh;Ou, Yu-cheng
    贡献者: 淡江大學機械與機電工程學系
    关键词: collagen;gelatin;glutaraldehyde;photosensitizing agent;protein;biocompatibility;biotechnology;cell adhesion;cell culture;cell density;cell growth;chemical structure;conference paper;controlled study;cross linking;culture medium;human;human cell;hydrophilicity;mesenchyme;particle size;photolithography;priority journal;robotics;stem cell;surface property;technique;Biocompatible Materials;Cell Culture Techniques;Cross-Linking Reagents;Gelatin;Glass;Glutaral;Humans;Mesenchymal Stem Cells;Nanotechnology
    日期: 2005-08
    上传时间: 2013-03-12 11:23:59 (UTC+8)
    出版者: Cambridge: RSC Publications
    摘要: This paper proposes a novel technique for fabricating micro patterns of glutaraldehyde (GA)-crosslinked gelatin. It provides another means to crosslink gelatin other than using photo-sensitizing agents, and the micro patterns of GA-crosslinked gelatin can still be made successfully by accessing conventional photolithography. A much less toxic and increased biocompatible approach to strengthening the gelatin microstructures can be developed according to this idea. This paper also describes a potential methodology for using GA-crosslinked gelatin patterns as single-cell culture bases. The best spatial resolution of the micro gelatin bases can reach 10 µm, and the selective growing density of human Mesenchymal stem cells on the gelatin patterns surpasses the density on the glass substrate by 2–3 orders of magnitude.
    關聯: Lab on a Chip 5(9), pp.979-984
    DOI: 10.1039/b505193b
    显示于类别:[機械與機電工程學系暨研究所] 期刊論文

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