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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/42012


    Title: Development of a whole-organism model to screen new compounds for sun protection
    Authors: Wang, Yun-hsin;Wen, Chi-chung;Yang, Zhi-shiang;Cheng, Chien-chung;Tsai, Jen-ning;Ku, Chia-chen;Wu, Hsin-ju;陳曜鴻;Chen, Yau-hung
    Contributors: 淡江大學化學學系;淡江大學數學學系
    Keywords: EGCG;Fin;Tea;UV;Zebrafish
    Date: 2009-06
    Issue Date: 2010-08-10 09:50:19 (UTC+8)
    Publisher: Springer
    Abstract: We used zebrafish as a whole-organism model to screen new compounds for sun protection activity. First of all, we designed a series of UVB exposure experiments and recorded the phenotypic changes of zebrafish embryos. Results showed that 100 mJ/cm2 of UVB given six times separated by 30 min intervals is the best condition. Fin malformation (reduced and/or absent fin) phenotypes are the most evident consequences after exposure to UVB. Each fin was affected by UVB, including pelvic, ventral, caudal, and dorsal fin, but pelvic fin seemed to be the most sensitive target after UVB exposure. We furthermore carried out “prevention” and “treatment” experiments using green tea extract and/or (−)-epigallocatechin (EGCG) to test this whole-organism model by observing the morphological changes of all fins (especially pelvic fin) after UVB exposure. Effects of UVB, green tea extract and EGCG on fin development were assessed using the Kaplan–Meier analysis, log-rank test and Cox proportional hazards regression. Results showed that a zebrafish pelvic fin in the UVB + green tea (treatment) group is 5.51 (range from 2.39 to 14.90) times, one in the UVB + green tea (prevention) group is 7.04 (range from 3.11 to 18.92) times, and one in the 25 ppm of EGCG (prevention) group is 22.19 (range from 9.40 to 61.50) times more likely to return to normal fin than one in the UVB only group. On the basis of these observations, we believe this model is effective for screening the higher stability and lower toxicity of new compounds, such as small chemicals which are derivative from EGCG or other dietary agents for sun protection.
    Relation: Marine Biotechnology 11(3), pp.419-429
    DOI: 10.1007/s10126-008-9159-9
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article
    [Graduate Institute & Department of Mathematics] Journal Article

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