English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 57505/91036 (63%)
造訪人次 : 13423311      線上人數 : 293
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/42012


    題名: Development of a whole-organism model to screen new compounds for sun protection
    作者: Wang, Yun-hsin;Wen, Chi-chung;Yang, Zhi-shiang;Cheng, Chien-chung;Tsai, Jen-ning;Ku, Chia-chen;Wu, Hsin-ju;陳曜鴻;Chen, Yau-hung
    貢獻者: 淡江大學化學學系;淡江大學數學學系
    關鍵詞: EGCG;Fin;Tea;UV;Zebrafish
    日期: 2009-06
    上傳時間: 2010-08-10 09:50:19 (UTC+8)
    出版者: Springer
    摘要: We used zebrafish as a whole-organism model to screen new compounds for sun protection activity. First of all, we designed a series of UVB exposure experiments and recorded the phenotypic changes of zebrafish embryos. Results showed that 100 mJ/cm2 of UVB given six times separated by 30 min intervals is the best condition. Fin malformation (reduced and/or absent fin) phenotypes are the most evident consequences after exposure to UVB. Each fin was affected by UVB, including pelvic, ventral, caudal, and dorsal fin, but pelvic fin seemed to be the most sensitive target after UVB exposure. We furthermore carried out “prevention” and “treatment” experiments using green tea extract and/or (−)-epigallocatechin (EGCG) to test this whole-organism model by observing the morphological changes of all fins (especially pelvic fin) after UVB exposure. Effects of UVB, green tea extract and EGCG on fin development were assessed using the Kaplan–Meier analysis, log-rank test and Cox proportional hazards regression. Results showed that a zebrafish pelvic fin in the UVB + green tea (treatment) group is 5.51 (range from 2.39 to 14.90) times, one in the UVB + green tea (prevention) group is 7.04 (range from 3.11 to 18.92) times, and one in the 25 ppm of EGCG (prevention) group is 22.19 (range from 9.40 to 61.50) times more likely to return to normal fin than one in the UVB only group. On the basis of these observations, we believe this model is effective for screening the higher stability and lower toxicity of new compounds, such as small chemicals which are derivative from EGCG or other dietary agents for sun protection.
    關聯: Marine Biotechnology 11(3), pp.419-429
    DOI: 10.1007/s10126-008-9159-9
    顯示於類別:[化學學系暨研究所] 期刊論文
    [數學學系暨研究所] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    1436-2228_11(3)p419-429.pdf331KbAdobe PDF400檢視/開啟
    Development of a whole-organism model to screen new compounds for sun protection.pdf331KbAdobe PDF0檢視/開啟
    index.html0KbHTML125檢視/開啟
    index.html0KbHTML17檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋