English  |  正體中文  |  简体中文  |  Items with full text/Total items : 56829/90592 (63%)
Visitors : 12146109      Online Users : 58
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/42011

    Title: A novel phenotype-based approach for systematically screening antiproliferation metallodrugs
    Authors: Wang, Yun-hsin;Cheng, Chien-chung;Lee, Wen-jie;Chiou, Min-lun;Pai, Chiung-wen;Wen, Chi-chung;Chen, Wei-li;Chen, Yau-hung
    Contributors: 淡江大學生命科學研究所
    Keywords: Apoptosis;Cisplatin;Fin;Metallodrug;Proliferation;Ruthenium
    Date: 2009-11-10
    Issue Date: 2010-02-23 14:03:13 (UTC+8)
    Publisher: Shannon: Elsevier Ireland Ltd
    Abstract: Ruthenium (Ru) derivatives have less toxicity and higher water-solubility than cisplatin, giving them great potential as antitumor metallodrugs. In this study, zebrafish were employed as a whole-organism model to screen new Ru compounds for anti-cell proliferation activity. After soaking fish embryos in cisplatin and five Ru derivatives, [Ru(terpy)(bpy)Cl]Cl, [Ru(terpy)(dppz)OH2](ClO4)2, [Ru(terpy)(tMen)OH2](ClO4)2, [Ru(terpy)(Me4Phen)OH2](ClO4)2, and Ru(bpy)2Cl2, only cisplatin and [Ru(terpy)(bpy)Cl]Cl-treated embryos displayed obvious phenotypic effects, such as fin-reduction. After further modification of [Ru(terpy)(bpy)Cl]Cl's main structure and the synthesis of two structurally related compounds, [Ru(terpy)(dcbpyH2)Cl]Cl and [Ru(terpy)(dmbpy)Cl]Cl, only [Ru(terpy)(dmbpy)Cl]Cl exhibited fin-reduction phenotypes. TUNEL assays combined with immunostaining techniques revealed that treatment with cisplatin, [Ru(terpy)(bpy)Cl]Cl, and [Ru(terpy)(dmbpy)Cl]Cl led proliferating fin mesenchymal cells to undergo apoptosis and consequently caused fin-reduction phenotypes. Furthermore, [Ru(terpy)(bpy)Cl]Cl was able to activate the P53-dependent and independent pathways, and induced human hepatoma cells to undergo apoptosis. In summary, it was concluded that the zebrafish model was effective for the screening of phenotype-based antiproliferation metallodrugs.
    Relation: Chemico-Biological Interactions 182(1), pp.84-91
    DOI: 10.1016/j.cbi.2009.08.005
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article
    [Graduate Institute & Department of Mathematics] Journal Article

    Files in This Item:

    File Description SizeFormat

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback