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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/42010

    Title: Transgenic zebrafish line with over-expression of Hedgehog on the skin: a useful tool to screen Hedgehog-inhibiting compounds
    Authors: Chen, Yau-hung;Wang, Yun-hsin;Yu, Tsung-han;Wu, Hsin-ju;Pai, Chiung-wen
    Contributors: 淡江大學生命科學研究所
    Keywords: Basal cell carcinoma;Keratin;Skin cancer;Sonic hedgehog;Transgenic fish;Zebrafish
    Date: 2009-12
    Issue Date: 2010-02-23 14:01:33 (UTC+8)
    Publisher: Dordrecht: Springer Netherlands
    Abstract: We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histology revealed embryos derived from Tg(k18:shh:RFP) displayed an elevated Nuclear/Cytoplasmic ratio and pleomorphic nuclei compared to their wild type littermates, suggesting the abnormal growth pattern on the epidermis of Tg(k18:shh:RFP) embryos were dysplasia. Later (by 7 dpf), Tg(k18:shh:RFP) embryos displayed broader pectoral fins which are similar to the polydactyly phenotypes of Nevoid basal cell carcinoma syndrome (NBCCS)/Gorlin patients and polydactylous mice. In addition, treatment with cyclopamine is able to enhance and prolong the survival rates and survival durations of Tg(k18:shh:RFP) embryos. In conclusion, this unique Tg(k18:shh:RFP) fish line, should be an excellent experimental animal for screening for a lower toxicity level of the new Hh-inhibitor and can even be used as a new anti-cancer drug-screening platform.
    Relation: Transgenic Research 18(6), pp.855-864
    DOI: 10.1007/s11248-009-9275-y
    Appears in Collections:[化學學系暨研究所] 期刊論文

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