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    題名: Multiple regulatory elements mediating neuronal-specific expression of zebrafish sodium channel gene, scn8aa
    作者: Wu, Si-huei;陳曜鴻;Chen, Yau-hung;Huang, Fong-lee;Chang, Chuan-hsiung;Chang, Yi-feng;Tsay, Huey-jen
    貢獻者: 淡江大學化學學系
    關鍵詞: zebrafish;scn8aa;GFP;transgenic;neuronal-specific
    日期: 2008-09-01
    上傳時間: 2010-08-10 09:52:22 (UTC+8)
    出版者: Wiley-Blackwell
    摘要: Zebrafish scn8aa sodium channels mediate the majority of sodium conductance, which is essential for the embryonic locomotor activities. Here, we investigated the transcriptional regulation of scn8aa in developing zebrafish embryos by constructing a GFP reporter driven by a 15-kb fragment of scn8aa gene designed as scn8aa:GFP. GFP expression patterns of scn8aa:GFP temporally and spatially recapitulated the expression of endogenous scn8aa mRNA during zebrafish embryonic development, with one exception in the inner nuclear layer of the retina. Three novel elements, along with an evolutionarily conserved element shared with mouse SCN8A, modulated neuronal-specific expression of scn8aa. The deletion of each positive element reduced the expression levels in neurons without inducing ectopic GFP expression in non-neuronal cells. Our results demonstrate that these four regulatory elements function cooperatively to enhance scn8aa expression in the zebrafish nervous system.
    關聯: Developmental Dynamics 237(9), pp.2554-2565
    DOI: 10.1002/dvdy.21680
    顯示於類別:[化學學系暨研究所] 期刊論文

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