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    題名: Effect of cell membrane structure of human erythrocyte on its electrophoresis
    作者: Hsu, Jyh-ping;Lin, Sung-hwa;曾琇瑱;Tseng, Shio-jenn
    貢獻者: 淡江大學數學學系
    關鍵詞: Cell membrane structure;Human erythrocyte;Electrophoresis;Mathematical model
    日期: 2003-11-01
    上傳時間: 2010-01-28
    出版者: Elsevier
    摘要: The electrophoretic behavior of human erythrocyte is investigated theoretically. The present analysis extends previous studies to that the effect of the membrane structure of a cell is taken into account. The result obtained provides a useful tool for the estimation of the surface properties of human erythrocyte through electrophoretic measurements. Fitting the present model to the experimental data of Haydon and Seaman [Arch. Biochem. Biophys. 122 (1967) 126], for example, reveals that the surface charge of erythrocyte arises mainly from the dissociation of three functional groups, which bond to different sites on cell membrane : (a) N-acetylneuraminic acid (sialic acid) with pKHANaa=2.6, (b) some kind of α-carboxylic acid with pKHAα−ca=3.35, (c) weak amino base functional group with pKBH+=8.5. The distribution of Na+ in the protein layer of a cell is found to vary with the pH of the bulk liquid phase, the possible implication of this phenomenon to cell physiology is discussed.
    關聯: Colloids and Surfaces B: Biointerfaces 32(3), pp.203-212
    DOI: 10.1016/S0927-7765(03)00158-9
    顯示於類別:[數學學系暨研究所] 期刊論文

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