近年來隨著石油價位的飆漲，造成經濟的波動，使人回憶起過去石油危機的憂患意識，因此有許多國家、公司和學校投資了龐大的人力與金錢，進而尋找開發新能源技術，其中直接甲醇燃料電池在可攜式電子電力產品之應用便是發展重點之一。 本研究目的是探討如何設計出一個符合穩定且高效率並具有實用性的直接甲醇燃料電池，吾人首先發展一套自動化測量系統，該系統包含量測與控制系統兩部份，其中量測系統主要在於自動化量測與紀錄電壓、電流、功率及溫度等實驗參數，控制系統則是自動化控制溶液加熱溫度、陰極氧氣供應、環境溫度控制及直流負載機等功能。 而研究的第二部份是將所設計量測平台，對單一燃料電池分別就環境與濃度實驗進行分析與探討，其中環境實驗方面會配合田口實驗法尋求較佳的環境操作條件，進行濃度實驗則是用多種濃度設定條件，探討較佳的燃料濃度。 最後本研究會使用計算流體力學電腦輔助工程分析軟體ICEPAK和FLUENT，針對直接甲醇燃料電池的陽、陰極流道做更進一步的系列探討與設計。陽極端設計的目的，是要讓燃料能夠盡可能的分佈均勻，若發生不均勻的現象會降低電池整體性能。陰極端空氣流道設計目的，在於陰極端氧氣供應過程中其風速、風壓與表面溫度需具有均勻性。本研究的最終目的在於結合實驗與模擬，提供一個有效的方法，使的直接甲醇燃料電池得以在適合的操作條件下，獲得較佳的性能。 An effective alternate energy resource has been urgent acquired because of the energy crisis in the world. Therefore, many country, company, or institutes invest a lot of people and money in the development of new energy technologies. The direct methanol fuel cell (DMFC) is prominent to be highly considered the application in the future portable electronics. The mainly object of this thesis is to illustrate how to design a stable, high performance, and practical DMFC. The first part of this paper is to develop an automatic diagnostic system. The developed system consists of measuring and control parts. The information of voltage, current, power, and temperature of the DMFC could be measured and recorded by the system; the system could also make the controls of the fuel inlet temperature, cathode airflow inlet environment temperature, and DC loading machine. The second part of this paper is to make the parametric studies on the operating environment as well as the concentration effect to a single-cell DMFC by utilizing the diagnostic system. The environment studies adopted the Taguchi’s method to figure out a better operation condition. The comparison of a DMFC performance under several different methanol concentrations at several specific operating conditions was conducted to get a good concentration range. In addition, the last part of the thesis is to apply the computational fluid dynamic technique to make a series of designs on the anode and cathode side flow channels. The design on the anode side emphasized the uniformity of the fuel supplying to the cells in the planar DMFC stack, where the non-uniformity could significantly decrease the DMFC performance. The design on the cathode side focused on the uniformity of the airflow velocity, pressure, and temperatures aside of the cells. The ultimate purpose of the research is to provide the methodology to make a good DMFC design under proper operating conditions by integrating the experimental and simulation results.