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    題名: 薄膜表面形態對微粒子/蛋白質雙成份懸浮液掃流微過濾之影響
    其他題名: Effects of membrane surface morphology on the performance of cross-flow microfiltration of fine particle/protein binary suspension
    作者: 邱鴻勝;Chiu, Hung-sheng
    貢獻者: 淡江大學化學工程與材料工程學系碩士班
    黃國楨;Hwang, Kuo-jen
    關鍵詞: 掃流微過濾;蛋白質純化;薄膜結垢;生化分離;cross-flow microfiltration;protein purification;fouling;bio-separation
    日期: 2005
    上傳時間: 2010-01-11 05:42:28 (UTC+8)
    摘要: 本研究探討薄膜形態與操作條件對薄膜結垢(fouling)的影響。實驗中以掃流微過濾來分離聚甲基丙烯酸甲酯/牛血清蛋白雙成份懸浮液,並以兩種表面形態不同之薄膜(Isopore與Durapore薄膜)進行實驗,探討掃流速度、過濾壓差、pH值及薄膜形態對濾速的影響;且針對粒子在濾材表面之附著機構加以研究。研究顯示,過濾阻力之來源主要為濾餅阻力所主導。此外,當pH值接近電位點(pI = 4.5)時,溶液中的粒子會凝聚成粒子團,進行過濾操作時會使濾速增加。為了方便探討粒子在薄膜孔道內形成阻塞,故將溶液環境維持在中性條件下。經由SEM圖可觀察到粒子阻塞及堆積在薄膜表面的情形,藉由理論的分析與實驗的結果,可模擬粒子在濾膜孔道中運動的軌跡,提供日後濾膜選擇與操作參數設定時的參考。
    The effects of membrane morphology and operating conditions on the filtration rate and membrane fouling are studied. Two kinds of membrane, Isopore and Durapore are used to separate the PMMA/BSA binary suspension cross-flow microfiltration. The filtration rates and the membrane fouling under various conditions, such as cross-flow velocity, pressure drop, pH values and membrane morphology, are measured and discussed. The experimental results show that the cake resistance is dominant in the overall filtration resistance. The coagulation occurred at the isoelectric point of BSA (pH 4.5) causes the filtration rate to be higher than the other suspension pH conditions. The pH of suspension is then kept at 7.0 in order to discuss the fouling phenomena in the membrane pores. The blocking and fouling phenomena of particles are observed by SEM, …. Further, the particle trajectory in the membrane pores is simulated by the theoretical analysis based on experimental results. These results can be used to select the optimum operating condition and correct filter membrane.
    顯示於類別:[化學工程與材料工程學系暨研究所] 學位論文

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