研究欲利用生物相容性佳與無毒性的天然高分子幾丁聚醣(Chitosan)作為主要材料，加入不同粒徑大小的三鈣磷酸鹽(Tricalcium phosphate；β-TCP)混合成複合材料，藉由β-TCP不斷釋放出鈣離子來引導骨性細胞吸附及加速骨組織的再生，同時亦藉著β-TCP來改善幾丁聚醣的機械性質。除了機械性質外，不同的薄膜結構將會對細胞的貼附遷移、分化及成長有所影響，因此利用乾式製程與冷凍乾燥配合乾式製程的製備不同結構之幾丁聚醣/β-TCP薄膜，再進行形態觀察、結晶構造分析、孔隙度測定、機械性質測試、膨潤率、生物降解性等，並以in vitro細胞培養的方式來評估幾丁聚醣/β-TCP薄膜的生物相容性。幾丁聚醣添加磷酸三鈣經由冷凍乾燥配合乾式製程以及乾式製程可得不同結構之薄膜，乾式製程所製備的薄膜，由SEM觀察為緻密結構的形態，添加不同粒徑的TCP於薄膜中，發現研磨後 的TCP顆粒(簡稱gTCP)分散於薄膜中時較為均勻。經冷凍乾燥配合乾式製程的薄膜結構，經由SEM觀察發現，隨著預熱處理時間的增加薄膜表面會越來越緻密，且預熱處理時間增加對於薄膜的整體厚度、孔隙度以及澎潤程度都有減小的趨勢，但機械性質卻隨之增加。當添加不同粒徑大小的TCP於薄膜中，經由SEM觀察薄膜的結構並沒有太大的改變，但薄膜添加TCP顆粒後對於薄膜的孔隙度與吸水程度均有減小的趨勢，而動態機械性質則因三鈣磷酸鹽的添加有增加的趨勢。在酵素水解測試中，TCP與gTCP的添加會降低其重量損失，且乾式製程製備的緻密薄膜的水解速率較凍乾薄膜慢。在生物相容性方面，L929細胞株對於不同結構的薄膜，均有正常細胞增殖的現象，而且各組之間並無明顯差距，此外，gTCP的添加對於L929細胞增殖有明顯的提升。對於hFOB1.19細胞株，乾式法之緻密薄膜與未經預烘處理的多孔狀薄膜具有較高的細胞增殖率，此外，添加gTCP於幾丁聚醣中，也有助於提高hFOB1.19的細胞增殖。 The aim of this study was to prepare a chitosan/β-tricalcium phosphate(CS/β-TCP) membrane where different particle sizes of tricalcium phosphate were used. The β-TCP can improve mechanical properties of chitosan and release Ca2+ ion to guide proliferation of osteoblastic cells. Different structures of membranes will affect the growth of cell. Therefore, the membrane fabricated by two methods. One is drying method and another is combining pre-drying with lyophilization. Different structures of CS/β-TCP membranes were observed using SEM and XRD methods and mechanical properties, swelling behaviors and biodegradable of composite membranes were measured. Finally, the cell culture of the fibroblastic cells and osteoblastic cells on these composite membranes was evaluated. In result, the structure of membrane was dense in using drying method. Added different particle sizes of tricalcium phosphate in membrane. It can find that the grinded tricalcium phosphate particles were uniform distributing in chitosan membrane. The structures of membranes were porous in combining pre-drying with lyophilization. It can find that were more dense in top surface of membranes with increased pre-heating time. With increased pre-heating time, the thickness, porosity and swelling of membranes were decreased. But the storage modulus were increased. It wasn’t effect structures of membranes with added tricalcium phosphate but it was decreased in porosity and swelling of membranes. And the storage modulus was increased with added tricalcium phosphate. In biodegradable test, tricalcium phosphate would decrease weight loss of membranes. L929 and hFOB1.19 has good viability in different structures of membranes. It can improve viability of cell with added grinded tricalcium phosphate.
