本研究以相轉換法製備生物分解性高分子－聚丁基烷酯Poly(3-hydroxybutyrate)薄膜，將其表面改質後開發出離胺酸/PHB、幾丁聚醣/PHB複合薄膜，並探討其在生醫方面的應用。複合薄膜之製作乃合併電漿誘導聚合與固定化兩種技術：首先將PHB薄膜表面電漿接枝聚丙烯酸或聚甲基丙烯酯環氧丙烷，然後將離胺酸與幾丁聚醣固定於薄膜表面。分別將不同孔隙結構及離胺酸與幾丁聚醣固定量之薄膜，用於骨母細胞與纖維母細胞之培養與革蘭氏陰性菌Escherichia coli.及陽性菌Bacillus cereus之抗菌測試，結果顯示離胺酸、幾丁聚醣固定量增加，細胞生長活性提高，且抗菌性也增強，其中以多孔型PHB薄膜表面固定幾丁聚醣時MTT吸收值及細胞貼附數量有最明顯的提升，並對E. coli及B. cereus均可達到近85%的抗菌活性。 Microporous membranes of a biodegradable polymer, poly(hydroxybutyric acid) (PHB), were prepared by a phase-inversion process. Moreover, PHB membranes were used to prepare lysine/PHB and chitosan/PHB composite membranes. These membranes were then applied in the biomedical field. The techniques of plasma induced polymerization and immobilization were employed to prepare the composite membranes. First, poly(acrylic acid) and poly(glycidyl methacrylate) were grafted on PHB membranes by plasma induced polymerization, then lysine and chitosan was immobilized by reacting with PAA or PGMA. Membranes with different porous structures and/or immobilize lysine and chitosan yields were used in culture of osteoblast cells and antibacterial tests of gram-negative (Escherichia coli), gram-positive (Bacillus cereus) bacteria. The biocompatibility and antibacterial activities were found to increase with increasing amount of lysine and chitosan on the membrane, for which porous membrane immobilized lysine and chitosan improved most in terms of cell MTT absorption and quantity of attachment. Also, an antibacterial activity of ~85% for both E. coli and B. cereus was achieved by this membran
