第二部份： 本實驗以高效能液相層析儀（HPLC）結合Photodiode Array (PDA) UV/Vis 偵測器，來進行不同產地中草藥紫苑的分析。將紫苑樣品的萃取液，以梯度沖提方式進行分離，比對不同波長（210、230、254、280nm）所偵測到的圖譜，由分析結果，選擇254nm作為最適合用來製作化學指紋圖譜的波長，並標定出相對比例含量較高的吸收波峰作為主要特徵波峰，藉由主要特徵波峰面積的差異性來建立化學指紋圖譜，及比較不同產地對於紫苑成分的影響。最後，再將實驗數據制定成化學指紋圖譜及雷達圖譜，此化學指紋圖譜如同藥材指紋身分證，以作為藥材品種、產地之參考依據。 PartⅠ A novel ion chromatographic method has been developed for the simultaneous analysis of the inorganic anions (iodate, bromate, nitrite, bromide and nitrate) in their mixture. The method is based on the ion-pair chromatographic separation followed by the ultraviolet absorption detection. The separation was achieved on the reverse phase octadecylsilica (ODS) column and acetonitrile-TBA-CH3COOH mobile phase containing tetra-n-butylammonium salt (TBA) as an ion-pairing reagent. The pH of the mobile phase, the optimal wavelength, the concentration of the ion-pairing reagent and the concentration of the organic solvent in the mobile phase are the important factors which affect the retention time and efficiency of the separation. Therefore, the optimal analytic method which was found out and established can provide a fast and efficient method for the simultaneous analysis of the inorganic anions.
PartⅡ Our aim in this study is that by utilizing the high performance liquid chromatography combined with the photodiode array (PDA) UV/Vis detector for analyzing the Chinese herb “Ziyuan” which were came from different producing areas. The extracts of the Chinese herb Ziyuan were separated by the gradient elution method and then the chromatograms obtained were compared with the different wavelength 210nm, 230nm, 254nm, 280nm. According to the results, the chromatograms of 254nm wavelength was selected and made the optimal chemical fingerprint chromatogram. Besides, there were several characteristic peaks chosen by the different peak area. The characteristic peaks were established the chemical fingerprint atlas and compared the composition of the Chinese herb Ziyuan which were came from different producing areas. Finally, the data obtained from the chromatograms were made the chemical fingerprint and radar atlas of Chinese herb. This radar atlas is like the identification card of the Chinese herb which could provide the information of their sources and producing areas.
