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https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/32788
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| Title: | 微晶片電泳結合電化學安培法在抗壞血酸存在下同時偵測乙醯氨酚、異黃嘌呤與尿酸 |
| Other Titles: | Simultaneous determination of acetaminophen, oxypurinol and uric acid in the presence of ascorbic acid with microchip |
| Authors: | 邱怡華;Chiu, Yi-hua |
| Contributors: | 淡江大學化學學系碩士班
林孟山;Lin, Meng-shan |
| Keywords: | 微晶片電泳;電化學;microchip;PDMS;electrochemical detection |
| Date: | 2009 |
| Issue Date: | 2010-01-11 02:45:29 (UTC+8) |
| Abstract: | 本研究將PDMS (polydimethylsiloxane)微晶片電泳與電化學偵測系統結合。於微流道晶片所使用的材質中，PDMS是目前被廣為應用的高分子聚合物之一，其具有低成本、透光性佳且有良好的機械性與可塑性等特性，然而運用於微晶片電泳中，其電滲透流不穩定以及親水性不佳等缺點，是過去文獻中不斷研究改善的部分；相較於常見的PDMS表面改質方式，如共價鍵修飾、電漿處理、動態塗佈與化學氣相沉積等，本研究以摻雜PU (Polyurethane) 的修飾方式，來達到較為簡易、快速且穩定的改質的目的。
透過偵測乙醯氨酚、異黃嘌呤與尿酸的實驗結果證實，經過PU改質後的PDMS微流道晶片，改善了PDMS電滲透流不穩定與親水性不佳的缺點，提升了樣品的分離效率。在系統最佳化條件下，樣品分離的解析度由未修飾的0.87提升到修飾有0.0075% PU的2.2，達到完全分離，而三種樣品的線性範圍分別為乙醯氨酚5 μM ~ 1 mM (R=0.999)，靈敏度0.130 nA / μM；異黃嘌呤10 μM ~ 1 mM (R=0.999) ，靈敏度0.054 nA / μM與尿酸20 μM ~ 1 mM(R=0.999)，靈敏度0.043 nA / μM；實驗重複20次操作對100μM之乙醯氨酚、異黃嘌呤與尿酸的偵測，所得之相對標準偏差分別為2.1%、3.5%以及3.3%。而干擾物抗壞血酸亦成功的以抗壞血酸氧化酶去除。
The low cost, elasticity, plasticity and high transparency of PDMS (polydimethylsiloxane) has been used extensively. However, the instable EOF (electroosmotic flow) and hydrophobic nature of PDMS limits the applications in microchip electrophoresis. A number of methods had been published in order to improve these shortcomings of PDMS, such as covalent modification, air plasma treatment, dynamic coating, and chemical vapor deposition. In this research, we make use of intermixing PU (Polyurethane) and PDMS to attain to simple, quick, and stable modification methods.
We demonstrate that a feasibility scheme to enhance the EOF and hydrophilicity by means of PU modified PDMS. Acetaminophen, oxypurinol and uric acid were measured by using amperometry with microchip electrophoresis. In the process of experiment, sample resolution rises from 0.87 of native PDMS to 2.2 of the optimum 0.0075% PU modified PDMS. According to optimum operation conditions, the linear ranges of acetaminophen, oxypurinol and uric acid are obtained between 5 μM ~ 1 mM (R=0.999), 10 μM ~ 1 mM (R=0.999) and 20 μM ~ 1 mM(R=0.999), respectively. The sensitivities of three samples are 0.131 nA / μM for acetaminophen, 0.054 nA / μM for oxypurinol and 0.043 nA / μM of uric acid. The relative standard deviation of twenty repetitive detections is 2.1% for acetaminophen, 3.5% for oxypurinol and 3.3% for uric acid. The interference ascorbic acid is eliminated from ascorbate oxisase. |
| Appears in Collections: | [化學學系暨研究所] 學位論文
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