| 摘要: | 本研究藉由改變溫度的培養或使用不同的誘導因子，找出誘導生產蛋白質的最適化條件，以Eschericha coli合成的重組蛋白質OraSE及GlmE為探討對象。OraSE蛋白質可先後經由疏水性管柱及金屬螯合親和層析管柱分離，得到純的蛋白。另一蛋白質GlmE，也可經由疏水性管柱及陰離子交換管柱分離，達到純化的目的。並比較在不同溫度方式及誘導因子的培養下，所獲得蛋白質產量的差異。
Optimization of the production for recombinant proteins, OraSE and GlmE, was investigated by using several defferent growth conditions in this stusy . OraSE protein was purified by Phenyl – Sepharose HP and Ni2+ - NTA column ; GlmE protein was purified by Phenyl – Sepharose HP and Q – Sepharose FF. Significant difference in protein yield was observed when induction temperature and inducer were changed . |