幾丁聚醣之鳳梨酵素水解產物於抗腫瘤及抗菌之效果探討

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題名:	幾丁聚醣之鳳梨酵素水解產物於抗腫瘤及抗菌之效果探討
其他題名:	The inhibitory effects of bromelin hydrolyzed chitosan on tumor cells and microorganisms
作者:	曾惠婷;Tzeng, Hui-ting
貢獻者:	淡江大學生命科學研究所碩士班王三郎;Wang, San-lang
關鍵詞:	幾丁質;幾丁聚醣;幾丁寡醣;幾丁寡醣分離法;腫瘤細胞存活率試驗;鳳梨酵素;chitinc;hitosan;chitooligosaccharides;chitooligosaccharide separating method;tumor cell viability test;bromelin
日期:	2005
上傳時間:	2010-01-11 02:30:03 (UTC+8)
摘要:	本研究主要探討幾丁質、幾丁聚醣物質，經由鳳梨酵素水解，所生成幾丁寡醣對於細菌、黴菌、腫瘤細胞之抑制效果。將2％烏賊軟骨粉末於pH5、37℃，經0.1%鳳梨酵素水解兩天，所得水解物具有最高產量，對於Fusarium oxysporum具有1.6U的抑制活性。將此水解物利用幾丁寡醣分離法回收得到幾丁寡醣，進行腫瘤細胞存活率試驗，結果顯示對於人類白血病細胞U937剩下69%的存活率。將0.1%水溶性幾丁聚醣在pH5、37℃，經0.1%鳳梨酵素水解一天，所得水解物具有最高產量。其對Bacillus subtilis具有抑制效果，亦對Aspergillus fumigatus具有0.8U的抑制活性。將此水解物利用幾丁寡醣分離法回收得到幾丁寡醣，進行腫瘤細胞存活率試驗，結果顯示對於小鼠大腸癌細胞CT26剩下57%的存活率。 The major purpose of this research was to investigate the inhibitory effects of chitosan on bacteria, fungus, and tumor cells. An aqueous solution of 2% squid pen powder was hydrolyzed, at pH5 and 37℃, with 0.1﹪bromelain for two days to get the highest yield of hydrolysis product. Chitooligosaccharides were then recovered from this hydrolysis product by chitooligosaccharide separating method. The recovered chitooligosaccharides were used do assay its viability effect on the tumor cells tested. The result showed a 69% viability effect on leukemia cell U937. The chitooligosaccharides also showed a 1.6U inhibitory effect on Fasarium oxysporum. An aqueous solution of 0.1% chitosan was hydrolyzed, at pH5 and 37℃, with 0.1﹪bromelain for one day to get the highest yield of hydrolysis product. The resultant hydrolysis product showed inhibitory effect on Bacillus subtilis, 0.8U inhibitory activitiy to Aspergillus fumigatus, and 57﹪viability effect on colon cell CT26.
顯示於類別:	[生命科學研究所] 學位論文

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