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    Title: Pseudomonas spp.細菌發酵烏賊軟骨生產生物活性物質及其應用
    Other Titles: Production and applications of bioactive materials by pseudomonas spp. using squid pen as carbon/nitrogen source
    Authors: 徐琬涵;Hsu, Wan-han
    Contributors: 淡江大學生命科學研究所碩士班
    王三郎;Wang, San-lang
    Keywords: Pseudomonas sp.;烏賊軟骨;去蛋白;N-乙醯幾丁寡醣;Pseudomonas spp.;squid pen;deproteinization;N-Acetyl chitooligosaccharides
    Date: 2009
    Issue Date: 2010-01-11 02:29:02 (UTC+8)
    Abstract: 本研究係利用Pseudomonas aeruginosa K-187與Pseudomonas sp. TKU015發酵烏賊軟骨生產胜肽、寡醣等生物活性物質之探討。以含1% 烏賊軟骨粉、0.1% K2HPO4 及0.05% MgSO4.7H2O之100 mL液態培養基,分別在37℃及30℃進行搖瓶培養。在烏賊軟骨去蛋白方面,培養4天後,K-187所得之蛋白質去除率為68%,而TKU015能去除SPP中的幾丁質保留部分蛋白質,與0.1%市售木瓜酵素及鳳梨酵素比較,鳳梨酵素對烏賊軟骨有較高的蛋白質去除率,反應4天後之去除率為70%。以K-187與TKU015之發酵上清液與0.1% 鳳梨酵素共同反應4天後,蛋白質去除率可高達99%,而SPP幾近被完全水解,故測其上清液,分析幾丁寡醣組成,添加0.1% 鳳梨酵素於K-187共同發酵水解SPP可得1~5醣。此外於植物生長方面,TKU015與K-187之發酵上清液富含胺基酸、胜肽等對青江菜的生長明顯有幫助。綜合上述,可應用在胜肽、胺基酸及幾丁寡醣之生產。
    Pseudomonas aeruginosa K-187 and Pseudomonas sp. TKU015 can be used for fermentation of squid pen in production of bioactive materials. The culture condition for K-187 and TKU015 was composed of 1% squid pen powder (SPP) (w/v), 0.1 % K2HPO4 and 0.05 % MgSO4.7H2O, and incubated in 100 mL of liquid medium in shaking flasks at 37℃ and 30℃. For deproteinization tests, K-187 fermentation of squid pen powder showed protein removal of 68% after 4 days, and TKU015 could removed chitin and retained protein content in spp.
    The squid pen powder was treated with 0.1% commercial papain and bromelain as a comparison, the deproteinization rate in combinations of 1% Pseudomonas spp. culture supernatant and 0.1% bromelain was all 99% while the rate was 59% and 69% in 0.1% papin with Pseudomonas spp. only. The chitinase of K-187 and TKU015 combined with 0.1% bromelain can hydrolyze squid pen powder efficiently which may apply to the productions of peptides, amino acids and chitoligosaccharides by useful for biological applications. N-Acetylglucosamine (GlcNAc) to N-acetylchitopentose (GlcNAc)5 were also produced from the culture supernatant by using K-187 strain for four days fermentation with 0.1% bromelain. Besides, The fourth day culture supernatant of Pseudomonas spp. showed maximal activity of 4-6 fold growth enhancing effect on Brassica chinensis Linn weight.
    Appears in Collections:[生命科學研究所] 學位論文

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