本研究係利用Pseudomonas aeruginosa K-187與Pseudomonas sp. TKU015發酵烏賊軟骨生產胜肽、寡醣等生物活性物質之探討。以含1％ 烏賊軟骨粉、0.1％ K2HPO4 及0.05％ MgSO4．7H2O之100 mL液態培養基，分別在37℃及30℃進行搖瓶培養。在烏賊軟骨去蛋白方面，培養4天後，K-187所得之蛋白質去除率為68％，而TKU015能去除SPP中的幾丁質保留部分蛋白質，與0.1％市售木瓜酵素及鳳梨酵素比較，鳳梨酵素對烏賊軟骨有較高的蛋白質去除率，反應4天後之去除率為70％。以K-187與TKU015之發酵上清液與0.1％ 鳳梨酵素共同反應4天後，蛋白質去除率可高達99％，而SPP幾近被完全水解，故測其上清液，分析幾丁寡醣組成，添加0.1％ 鳳梨酵素於K-187共同發酵水解SPP可得1～5醣。此外於植物生長方面，TKU015與K-187之發酵上清液富含胺基酸、胜肽等對青江菜的生長明顯有幫助。綜合上述，可應用在胜肽、胺基酸及幾丁寡醣之生產。 Pseudomonas aeruginosa K-187 and Pseudomonas sp. TKU015 can be used for fermentation of squid pen in production of bioactive materials. The culture condition for K-187 and TKU015 was composed of 1% squid pen powder (SPP) (w/v), 0.1 % K2HPO4 and 0.05 % MgSO4．7H2O, and incubated in 100 mL of liquid medium in shaking flasks at 37℃ and 30℃. For deproteinization tests, K-187 fermentation of squid pen powder showed protein removal of 68% after 4 days, and TKU015 could removed chitin and retained protein content in spp. The squid pen powder was treated with 0.1% commercial papain and bromelain as a comparison, the deproteinization rate in combinations of 1% Pseudomonas spp. culture supernatant and 0.1% bromelain was all 99% while the rate was 59% and 69% in 0.1% papin with Pseudomonas spp. only. The chitinase of K-187 and TKU015 combined with 0.1% bromelain can hydrolyze squid pen powder efficiently which may apply to the productions of peptides, amino acids and chitoligosaccharides by useful for biological applications. N-Acetylglucosamine (GlcNAc) to N-acetylchitopentose (GlcNAc)5 were also produced from the culture supernatant by using K-187 strain for four days fermentation with 0.1% bromelain. Besides, The fourth day culture supernatant of Pseudomonas spp. showed maximal activity of 4-6 fold growth enhancing effect on Brassica chinensis Linn weight.
