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    Title: 發展新型電化學感測技術應用於研究甲型胎兒蛋白與其抗體間之免疫作用
    Other Titles: Development of a novel electrochemical technology for immunization between alpha fetoprotein and anti-alpha fetoprotein antibody
    Authors: 趙雅涵;Chao, Ya-han
    Contributors: 淡江大學生命科學研究所碩士班
    李世元;Lee, Adam Shih-yuan
    Keywords: 電化學分析術;甲型胎兒蛋白;流道式系統;Electrochemistry analysis;Alpha-fetoprotein;Flow-injection analysis
    Date: 2009
    Issue Date: 2010-01-11 02:27:18 (UTC+8)
    Abstract: 肝癌是現今為人熟知的疾病,根據行政院衛生署統計,肝癌為台灣地區癌症死亡率的第二位,發病半年後病人有極高機率死亡。本研究架構於電化學量測系統中,針對最常用的肝癌測試指標--甲型胎兒蛋白,開發新型電化學技術,並與血清蛋白比較,電化學分析較傳統ELISA分析有更快的即時性。由於肝癌腫瘤擴散迅速,而且發現不易,因此希望發展出兼具即時與高靈敏度的偵測技術。
    本實驗方法是固定甲型胎兒蛋白抗體於修飾之金電極上,同時依序接上0.1μg/mL、0.2μg/mL、0.5μg/mL 1μg/mL、10μg/mL 20μg/mL、50μg/mL、75μg/mL、100μg/mL,九個濃度的甲型胎兒蛋白,並個別在0V、-0.2V、-0.5V電壓下來測量不同濃度對平衡電流訊號所造成的變化,結果發現使用金作為參考電極在-0.5V下偵測,有最佳之線性範圍0.1 ~ 1μg/mL,線性回歸係數(R2)可達0.85,當接上濃度大於1μg/mL後,電流訊號便會慢慢趨緩,而濃度達到20ug/mL時,甲型胎兒蛋白抗體與抗原之間結合會有飽和現象。因此,選擇用金作為參考電極,並在-0.5V進行流道系統測試,結果發現量測相同濃度甲型胎兒蛋白可獲得高於靜態式系統數倍的電流訊號。
    Hepatocellular carcinoma are well-known disease nowadays. Counts according to the statistical information provided by the Department of Health, it’s the second leading cause of cancer deaths in Taiwan. In this study, Alpha-fetoprotein(AFP) is a common index of Hepatocellular carcinoma test. This thesis develops a detect technology for the electrochemistry. The performance of the proposed AFP is compared with the serum albumin. It is shown that electrochemical technology is faster than the ELISA. Hepatocellular carcinoma tumor invasion is very quick, and the discovery is not easy, therefore the develops has both immediate and the high sensitivity detection technology.
    The anti-AFP were immobilized on the gold electrode surface. The equilibrium current signal have measured by electrochemistry analysis Instrument under different concentration solutions (0.1μg/mL,0.2μg/mL,0.5μg/mL,1μg/mL,10μg/mL,20μg/mL, 50μg/mL,75μg/mL,100μg/mL). The result showed that the use gold takes the reference electrode to detect under -0.5V has the best linearity range(R2=0.85). The current signal difference will become slower after joining the concentration is more than 1ug/mL. When concentration is 20μg/mL, the association between antibody and antigen will be saturated. Therefore, the current signal have measured by flow injection analysis system under -0.5V. The result showed that the current signal is higher than the static system under same concentration.
    Appears in Collections:[生命科學研究所] 學位論文

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