本實驗主要針對二-(十-氫氧基癸烷基)丙二酸【2-(10-Hydroxydecyl)malonic acid】與金黃色葡萄球菌(Staphylococcus aureus)進行生物活性測試，並利用原子力顯微技術量測經二-(十-氫氧基癸烷基)丙二酸處理前後表面奈米結構暨生物力學之變化。 實驗條件為配製5 ppm、10 ppm、20 ppm、50 ppm及100 ppm濃度之二-(十-氫氧基癸烷基)丙二酸與金黃色葡萄球菌進行生物抑制反應。結果得知，濃度100 ppm之二-(十-氫氧基癸烷基)丙二酸可有效抑制金黃色葡萄球菌之生長。因此，選擇濃度100 ppm之二-(十-氫氧基癸烷基)丙二酸與金黃色葡萄球菌進行有效抑制時間測試，時間點分別於10分鐘、20分鐘、30分鐘、40分鐘、50分鐘、1小時、2小時、3小時、6小時、9小時、12小時及24小時取樣並進行細菌培養，結果發現時間在20分鐘內即可有效抑制金黃色葡萄球菌生長。為探討金黃色葡萄球菌經二-(十-氫氧基癸烷基)丙二酸處理前後之表面奈米結構暨生物力學變化，本篇研究選擇以原子力顯微技術進行表面奈米結構暨生物力學分析。 The differences of structural conformation and surface force on Staphylococcus aureus-ATCC 25923(S. aureus-25923) was treated with 2-(10-hydroxydecyl)malonic acid and were measured by atomic force microscope technology. Susceptibility to 2-(10-hydroxydecyl)malonic acid concentration was investigated with the population analysis technique in reference strains of S. aureus-25923. Comparisons of these results showed that S. aureus-25923 were inhibited by 2-(10-hydroxydecyl)malonic acid which concentration was approximately equal or higher than 100 ppm of the respective minimal inhibitory concentrations (MIC). Regarding the susceptibility to 2-(10-hydroxydecyl)malonic acid, there was a homogeneity of data with the respective time inhibit curves showing a clear bactericidal effect after 20 minutes of exposure. The results of susceptibility, respective time and force analysis were further applied to design a new medicine.
