本論文是使用一般X光的光源做一系列提高影像解析度及增強相位對比之研究。影像解析度方面,透過改變孔洞材質及大小、幾何放大倍率和空間同調長度,來得到綜合放大倍率及解析度的最佳條件。並以此最佳實驗解析條件,用已進一步觀察在一般水質生長的斑馬魚骨骼和在咖啡因濃度17.5ppm、35ppm及50ppm水質生長的斑馬魚骨骼有何異同。從實驗結果可看出咖啡因會造成斑馬魚的骨節萎縮、消失或扭曲。 在提高影像相位對比方面,利用Zernike之方法,分析所得之數據,並與F.Pfeiffer所使用之相位對比之成像系統相互比較。 This thesis bases on the common X-ray sources to capture and investigate a series of X-ray images and X-ray phase contrast images. In the part of images resolution , we could get the best condition of experiment through the changing material and size of pinhole, and also adjusting the magnification and the spatial coherence length. We applied the best condition of experiment to observe and compare the bones difference between normal zebra fish and experimental zebra fish which were growing up in 17.5ppm、35ppm and 50ppm caffeine water. From the result, we can find out caffeine will cause bones becoming atrophy, vanished or twist. For enhancing phase contrast images, we analyzed experimental data, and compare with F.Pffeier experiment by using Zernike’s phase contrast method.