淡江大學機構典藏:Item 987654321/28049
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 62819/95882 (66%)
造訪人次 : 4001345      線上人數 : 596
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋


    請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/28049


    題名: Identification of Peptides Using Gold Nanoparticle-Assisted Single-Drop Microextraction Coupled with AP-MALDI Mass Spectrometry
    作者: Sudhir, Putty-reddy;Wu, Hui-fen;周子聰;Zhou, Zi-cong
    貢獻者: 淡江大學物理學系
    日期: 2005-10-05
    上傳時間: 2009-12-31 11:00:37 (UTC+8)
    出版者: American Chemical Society (ACS)
    摘要: A novel technique, gold nanoparticle-assisted single-drop microextraction (SDME) combined with atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) for the identification of peptides has been described. The SDME of peptides from aqueous solution was achieved using gold nanoparticles prepared in toluene as the acceptor phase. A simple phenomenon of isoelectric point (pI) of the peptides has been utilized successfully to extract the peptides into a single drop of nanogold in toluene. After extraction, a single-drop nano gold solution was directly spotted onto the target plate with an equal volume of matrix, ∝-cyanohydroxy cinnamic acid (∝-CHCA) and analyzed in AP-MALDI-MS. The parameters, such as solvent selection, extraction time, agitation rate, and pH effect, were optimized for the SDME technique. Using this technique, in aqueous solution, the lowest concentration detected for Met- and Leu-enkephalin peptides was 0.2 and 0.17 μM, respectively. In addition, the application of this technique to obtain the signal for the selected peptides in a mass spectrum in the presence of matrix interferences such as 1% Triton X-100 and 6.5 M urea has been showed. The application was extended to identify the peptides spiked into urine.
    關聯: Analytical Chemistry 77(22), pp. 7380-7385
    DOI: 10.1021/ac051162m
    顯示於類別:[物理學系暨研究所] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    0KbUnknown324檢視/開啟
    Identification of Peptides Using Gold Nanoparticle-Assisted Single-Drop Microextraction Coupled with AP-MALDI Mass Spectrometry.pdf236KbAdobe PDF2檢視/開啟
    index.html館藏資訊0KbHTML159檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋